This is amino acids 796 to 815 fragment of the chicken gizzard smooth-muscle myosin light-chain kinase (smMLCKp or P7) peptide, calmodulin (CaM)-binding domain . It retains its affinity for CaM and represents an adequate model for a complex of CaM with intact smMLCK protein. In the globular (Ca2+)4-CaM-P7 complex, the protein is wrapped around the -helical peptide. All the lysine and arginine charges of P7 are in close contact with the negatively charged side chains of the acidic residues of CaM. Calmodulin operates as a Ca2+ sensor and is known to interact with and regulate hundreds of proteins involved in many aspects of cellular function.
Frederick, K. et al. Nature 448, 325 (2007); André. I. et al. Biophys. J. 90, 2903 (2006).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Arg - Arg - Lys - Trp - Gln - Lys - Thr - Gly - His - Ala - Val - Arg - Ala - Ile - Gly - Arg - Leu - Ser - Ser - OH