This substrate has a fluorescent Trp residue in subsite Pí2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-Pí1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-Pí1 and Pí3 is optimized for MMP-8, while Arg is placed in Pí4 to enhance solubility. Abs/Em = 280/360 nm.
Ref: Aschi, M. et al. J. Comput. Aided. Mol. Des. 16, 213 (2002); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
Dnp - Pro - Leu - Ala - Tyr - Trp - Ala - Arg - OH