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Assay Kits  >  Renin Assay Kits  >>  SensoLyte® 520 Rat Renin Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Rat Renin Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72140
US$ $687
Description

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research. The SensoLyte® 520 Rat Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. The kit contains: 5-FAM/QXL™520-based FRET rat renin substrate (Ex/Em=490/520 nm upon cleavage), assay buffer, rat recombinant renin, inhibitor, fluorescence reference standard for calibration, and an optimized assay protocol. Assays are performed in a convenient 96-well format.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Chelko, SP. et al. (2012). A novel vascular clip design for the reliable induction of 2-kidney, 1-clip hypertension in the rat. J Appl Physio 112, 362.
Cheng, W-H. et al. (2012). Renin activates PI3K-Akt-eNOS signaling through the AT1 receptor and Mas receptor to modulate central blood pressure control in the nucleus tractus solitarii. Brit J Pharma DOI: 10.1111/j.1476-5381.2012.01832.x.
Shah, N. et al. (2012). Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression. J Hepatol 56, 1047.
     
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