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Peptides  >  Bacterial Peptides  >>  Bacterial Sortase Substrate II , Dabcyl/Edans

Product Name Bacterial Sortase Substrate II , Dabcyl/Edans
Dabcyl - QALPETGEE - Edans
Size 1 mg
Catalog # AS-62230
US$ $264
Purity % Peak Area By HPLC ≥ 95%
Description

This peptide is a C-terminal surface sorting signal with a conserved LPXTG motif, labeled with the Dabcyl/Edans FRET pair. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Surface proteins of Staphylococcus aureus are anchored to the bacterial cell wall by a mechanism requiring this C-terminal sorting signal. Cell wall sorting is the covalent attachment of surface proteins to the peptidoglycan via a C-terminal sorting signal that contains a consensus LPXTG sequence. Cleavage of this FRET substrate by sortase reveals the fluorescent signal that may be measured to study sortase activity. Inhibition of the sortase activity is a potential way of treatment of the staphylococcal infection. The LPXTG motif is conserved in more than 100 surface proteins of Gram-positive pathogens.

Storage -20C
References Ton-That, H. et al. PNAS 96, 12424 (1999); W. W Navarre and O. Schneewind Mol. Biol. Rev. 63, 174 (1999); Kang, S. et al. Biol. Pharm. Bulletin 29, 1751 (2006); Ilangovan, U. et al. PNAS 98, 6056 (2001).
Molecular Weight 1472.6
Sequence
(One-Letter Code)
Dabcyl-QALPETGEE-Edans
Sequence
(Three-Letter Code)
Dabcyl - Gln - Ala - Leu - Pro - Glu - Thr - Gly - Glu - Glu - Edans
     
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