5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.
Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700; Hung SC, et al. (1996). Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers. Anal Biochem 243, 15-27; Banks PR and Paquette DM (1995). Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis. Bioconjug Chem 6, 447-458.
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