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Assay Kits  >  Calpain Assay Kits  >>  SensoLyte 520 Calpain Activity Assay Kit *Fluorimetric*

Product Name SensoLyte 520 Calpain Activity Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72149
US$ $501
Description

The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (μ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer's disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.

The SensoLyte 520 Calpain Activity Assay Kit is optimized for detecting calpain activity. This kit contains a novel internally quenched 5-FAM/QXL 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format.

Kit size: 100 assays

RELATED PRODUCT:
SensoLyte AMC Calpain Activity Assay Kit (Ex/Em=354/442 nm)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
Product Citations Jock, M. et al. (2014). Effect of partial outlet obstruction and reversal on rabbit bladder physiology and biochemistry: Duration of recovery and severity of function. BJU Intl doi: 10.1111/bju.12687.
Kawashima, I. et al. (2014). Targeted disruption of Nrg1 in granulosa cells alters the temporal progression of oocyte maturation. Mol Endo 28, 706.
Callaghan, CM. et al. (2013). The effect of partial outlet obstruction on calpain and phospholipase-2 activities: analyzed by severity and duration. Mol Cell Biochem 381, 217. doi: 10.1007/s11010-013-1705-8.
Kawashima, I. et al. (2012). EGF-Like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement. Endocrinol 153, 3949. doi: 10.1210/en.2012-1059.
Novgorodov, S. et al. (2011). Developmentally Regulated Ceramide Synthase 6 Increases Mitochondrial Ca2+ Loading Capacity and Promotes Apoptosis. J Biol Chem 286, 4644.
     
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