LRB - SC [Lissamine™ rhodamine B sulfonyl chloride]
LissamineTM rhodamine B (LRB) sulfonyl chloride (SC) is a mixture of isomeric sulfonyl chlorides. Its fluorescence emission spectrum lies between those of tetramethylrhodamines and X-rhodamines, and it can be well excited by 568 nm line of the Ar-Kr mixed gas laser that is used in many confocal laser-scanning microscopes. Like FITC reagents, LRB-SC is relatively inexpensive. However, it is quite labile in basic solutions, making it somewhat difficult to achieve reproducible conjugations.
-20°C desiccated and protected from light
Smith SN and Steer RP (2001). The photophysics of Lissamine rhodamine–B sulfonyl chloride in aqueous solution: implications for fluorescent protein–dye conjugates. J Photochem Photobiol, A 139, 151; Neves C, et al. (2000). Novel method for covalent fluorescent labeling of plasmid DNA that maintains structural integrity of the plasmid. Bioconjug Chem 11, 51-5.