HiLyte™ Fluor488-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. HiLyte™ Fluor488 is a green flurophore with brighter fluorescence and better photostability than fluorescein, such as FITC. The spectrum of HiLyte™ Fluor488 is similar to FITC, resulting in an optimal match to filters designed for fluorescein. The fluorescence of HiLyte™ Fluor488 can be observed at excitation/emission wavelength of 499 nm/523 nm.The goat anti-rabbit IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein. This secondary antibody is ideal for multi-fluorescence staining in which several primary antibodies from different species are involved.