HiLyte Fluor™ 405 is a new blue fluorescent dye with the structure designed for maximum brightness when conjugated to macromolecules. HiLyte Fluor™ 405-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. The fluorescence signal of HiLyte Fluor™405 can be observed with excitation and emission wavelengths of 404/428 nm.