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Assay Kits  >  Cathepsin Assay Kits  >>  SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72170
US$ $435
Description

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.

The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.

The kit contains:
• HiLyte™ Fluor 488/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage)
• Assay buffer
• Bovine spleen Cathepsin D
• Inhibitor
• Fluorescence reference standard for calibration
• A detailed protocol

Kit size: 100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
Product Citations Feng D. et al. (2011). High-Fat Diet–Induced Adipocyte Cell Death Occurs Through a Cyclophilin D Intrinsic Signaling Pathway Independent of Adipose Tissue Inflammation. Diabetes 60;2134
Li, PP. et al. (2010). Functional Heterogeneity of CD11c-positive Adipose Tissue Macrophages in Diet-induced Obese Mice. J. Biol. Chem 285:15333.
Buckow, R. et al. (2010). Bovine cathepsin D activity under high pressure. Food Chemistry 120;474.
     
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