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Assay Kits  >  Cathepsin Assay Kits  >>  SensoLyte® 520 Cathepsin G Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Cathepsin G Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72185
US$ $435
Description

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.

The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte™ Fluor 488/QXL® 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte™ Fluor 488/QXL® 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations.

The kit contains:
• HiLyte™ Fluor 488/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage)
• Assay buffer
• Human Cathepsin G
• Inhibitor
• Fluorescence reference standard for calibration
• A detailed protocol


Kit size: 100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
Product Citations Wågsäter, D. et al. (2012). Serine protease inhibitor A3 in atherosclerosis and aneurysm disease. Intl J Mol Med 30, 288.
     
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