Assay Kits  >  Cathepsin Assay Kits  >>  SensoLyte^® Rh110 Cathepsin G Assay Kit *Fluorimetric*

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs. The SensoLyte^® Rh110 Cathepsin G Assay Kit provides a convenient assay for high throughput screening of enzyme inhibitors or for continuous assay of enzyme activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496/520 nm.

The kit contains:
• Rh110 based fluorogenic substrate (Ex/Em=490/520 nm upon cleavage)
• Assay buffer
• Human Cathepsin G
• Inhibitor
• Fluorescence reference standard for calibration
• A detailed protocol

Kit size: 100 assays (96-well plate)