Sirtuins comprise a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) targeting multiple protein substrates. Sirtuin 2 (SIRT2) belongs to the family of Sir2 (Silent Information Regulator 2) proteins. Substrates for SIRT2 are not limited to histones but also include various transcription factors and co-regulators that modulate metabolic, cell cycle and cell death related pathways. Human SIRT2 is a cytoplasmic protein that increases in abundance during mitosis and regulates major events of cytokinesis. This human recombinant SIRT2 is in its active form, and can be used for enzyme activity assays.