This peptide is histone H3 (1-21) with symmetrical dimethylation at Arg2 and trimethylation at Lys4, followed by a C-terminal GGK with biotinylation on the side chain of Lys. Dimethylation of the guanidinium group of Arg2 is associated with transcriptional silencing. Trimethylation of Lys4, which is catalyzed by Set1, is associated exclusively with active euchromatic genes. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Musselman, CA and TG. Kutateladze Mol Interv9, 314 (2010); Kirmizis, A. et al. Nat Struct Mol Biol16, 449 (2009); Tamaru, H. et al. Nat Genet34, 75 (2003); Santos-Rosa, H. et al. Nature419, 407 (2002).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Arg(Me2s) - Thr - Lys(Me3) - Gln - Thr - Ala - Arg - Lys - Ser - Thr - Gly - Gly - Lys - Ala - Pro - Arg - Lys - Gln - Leu - Ala - Gly - Gly - Lys(Biotin) - OH