This peptide is histone H3 58-89 with dimethylation at Lys79 and biotin-labeled through a C-terminal GGK linker. Lys79 methylation is mediated by DOT1 protein. It has been shown that dimethylation at Lys79 is important for transcriptional activation via mixed lineage leukemia gene (MLL)-ENL-ER and MLL-AF9. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Milne, T. et al. Cancer Res65, 11367 (2005); Schubeler, D. et al. Gene Dev18, 1263 (2004); Feng, Q. et al. Curr Biol12, 1052 (2002).
H - Thr - Glu - Leu - Leu - Ile - Arg - Lys - Leu - Pro - Phe - Gln - Arg - Leu - Val - Arg - Glu - Ile - Ala - Gln - Asp - Phe - Lys(Me2) - Thr - Asp - Leu - Arg - Phe - Gln - Ser - Ala - Ala - Ile - Gly - Gly - Lys(Biotin) - OH