This peptide is histone H4 (1-25) with acetylation at Lys5, Lys8, Lys12, and Lys16, followed by a C-terminal GSGS linker and a biotinylated lysine. Acetylation at Lys5, Lys8, and Lys12 is carried out by p300 protein while acetylation at Lys16 is regulated by several histone acetyltransferases. These lysine residues have been shown to have important functions in transcriptional activation, replication, and nuclear division. Acetylation of lysine residues promotes binding of transcription factors to histone H4. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Vettese-Dadey, M. et al. EMBO J15/b>, 2508 (1996); Megee, PC et al. Science247, 841 (1990).