Species Reactivity: This antibody will recognize long variants of Tau (1N4R, 2N3R, and 2N4R) in human, monkey, mouse, rat, and other mammalian species. Antibody specificity was confirmed by Western blot. This antibody will recognize Tau Ser400 modifications including phosphorylation and glycosylation.
Application: ELISA, WB
Product Description: Rabbit polyclonal Anti-Tau Antibody (Ser400) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 µg/µl) Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Tau Antibody (Ser400) was raised against a synthetic peptide corresponding to the surrounding of Tau Ser400 residue without modifications.
Application Notes: The following concentration ranges are recommended ranges. Optimal working concentrations for specific application should be determined by the investigator.
WB: 1 μg/ml IHC (recommended but not tested): 1-2 μg/ml
Figure 1. Recombinant Tau (2N4R) and rat brain lysate were probed with Anti-Tau (Ser 400) antibody at 1 µg/ml final concentration. Secondary Goat Anti-Rabbit IgG-HiLyte Fluor™ 750 nm labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize fluorescent band in the near-infrared part of the light spectra. Multiple immunoreactive bands were detected for rat brain lysate and a single band was detected for recombinant Tau (as expected).