Anti Tau Antibody (GlcNAc Ser400), Rabbit PolyclonalNEW
Species Reactivity: This antibody will recognize glycosylated Tau in human, monkey, mouse, rat, and other mammalian species. Antibody specificity was confirmed by Western and dot blots. This antibody will not recognize non-glycosylated Tau.
Application: WB, Dot blot
Product Description: Rabbit polyclonal Anti-Tau Antibody (GlcNAc Ser400) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 µg/µl) Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.
Immunogen: Rabbit polyclonal Anti-Tau Antibody (Ser400) was raised against a synthetic peptide corresponding to the surrounding of Tau Ser400 residue and glycosylated at Serine400 residue with N-acetyl-D-glucosamine (GlcNAc).
Application Notes: The following concentration ranges are recommended ranges. Optimal working concentrations for specific application should be determined by the investigator.
WB: 1 μg/ml IHC (recommended but not tested): 1-2 μg/ml
An antibody raised against a synthetic peptide corresponding to the surrounding amino acids of Tau Ser400 residue (without modifications) is available. This antibody will recognize Tau Ser400 modifications including phosphorylation and glycosylation - Anti-Tau Antibody (Ser400), Rabbit Polyclonal
Figure 1. Recombinant Tau (2N4R) and rat brain lysate were probed with Anti-Tau (GlcNAc Ser 400) antibody at 1 µg/ml final concentration. Secondary Goat Anti-Rabbit IgG-HiLyte Fluor™ 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize fluorescent band in the near-infrared. Multiple immunoreactive bands were detected for rat brain lysate and no band was detected for recombinant Tau (as expected).
Figure 2. Tau (GlcNAc Ser 400) and non-glycosylated immunizing tau peptides was probed with Anti-Tau (GlcNAc Ser 400) antibody at 1 µg/ml final concentration. Secondary Goat Anti-Rabbit IgG-HiLyte Fluor™ 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize fluorescent dot in the near-infrared. This antibody does not react with "naked" Tau peptide.