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Antibodies  >  Primary Antibodies  >  Alzheimer's Disease  >>  Anti Tau Antibody (GlcNAc Ser400), Rabbit Polyclonal

Product Name Anti Tau Antibody (GlcNAc Ser400), Rabbit Polyclonal  NEW
Size 50 µg
Catalog # AS-55945
US$ $215
Description

Host: Rabbit

Species Reactivity: This antibody will recognize glycosylated Tau in human, monkey, mouse, rat, and other mammalian species. Antibody specificity was confirmed by Western and dot blots. This antibody will not recognize non-glycosylated Tau.

Application: WB, Dot blot

Product Description: Rabbit polyclonal Anti-Tau Antibody (GlcNAc Ser400) is supplied as an epitope affinity purified rabbit IgG, 50 μg in 250 μl (0.2 μg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.

Immunogen: Rabbit polyclonal Anti-Tau Antibody (Ser400) was raised against a synthetic peptide corresponding to the surrounding of Tau Ser400 residue and glycosylated at Serine400 residue with N-acetyl-D-glucosamine (GlcNAc).

Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.

WB: 1 μg/ml
IHC (recommended but not tested): 1-2 μg/ml


RELATED PRODUCTS:
An antibody raised against a synthetic peptide corresponding to the surrounding amino acids of Tau Ser400 residue (without modifications) is available. This antibody will recognize all Tau modifications including phosphorylation and glycosylation - Anti-Tau Antibody (Ser400), Rabbit Polyclonal

Tau Peptides - 6 isoforms & [Ser(OGlcNAc)]400-KWK-Tau (388-411)-KK(Biotin)-NH2

Product Spotlight: Tau Products - Antibodies, Recombinant Proteins and Peptides




Figure 1. Recombinant Tau (2N4R) and rat brain lysate were probed with Anti-Tau (GlcNAc Ser 400) antibody at 1 μg/ml final concentration. Secondary Goat Anti-Rabbit IgG-HiLyte Fluor™ 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize fluorescent band in the near-infrared. Multiple immunoreactive bands were detected for rat brain lysate and no band was detected for recombinant Tau (as expected).

Figure 2. Tau (GlcNAc Ser 400) and non-glycosylated immunizing tau peptides were probed with Anti-Tau (GlcNAc Ser 400) antibody at 1 μg/ml final concentration. Secondary Goat Anti-Rabbit IgG-HiLyte Fluor™ 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize fluorescent dot in the near-infrared. This antibody does not react with “naked” Tau peptide.
Images Anti Tau Antibody (GlcNAc Ser400), <i>Rabbit Polyclonal</i>
Detailed Information Datasheet
Storage -20°C
     
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