Anti - IRAK1 (pThr387) Antibody (Clone 2D6), mouse monoclonalNEW
Species Reactivity: Human, mouse, rat; others not tested.
Application: Dot blot
Product Description: Mouse monoclonal anti-IRAK (pThr387) (2D6) antibody is supplied as an epitope affinity purified mouse monoclonal antibody, 50 µg in 250 µl 1x PBS (pH 7.4) containing 0.02% sodium azide.
Immunogen: This mouse monoclonal antibody was raised against a synthetic peptide corresponding to human interleukin-1 (IL-1) receptor-associated kinase (IRAK-1), phosphorylated at threonine 387 (Accession number: NP_001020414).
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific applications should be determined by the investigator.
Dot Blot: 1: 200-2,000 WB (recommended but not tested): 1: 200-2,000 IHC (recommended but not tested): 1:100-1,000 Immunoprecipitation (recommended but not tested): 3.0-5.0 µg/extract from 107 cells
Figure 1. Dot blot of mouse anti-IRAK-1 (pThr387) monoclonal antibody using synthetic peptides: IRAK-1 (Phosphorylated Thr387), and IRAK-1 (Thr387) at 0.02, 0.2 and 1 µg. Primary antibody was then probed with Goat anti-mouse IgG (H+L), highly cross-adsorbed, HiLyte Fluor™ 750-labeled (Cat# 61057-1-H750) for signal detection.