Species Reactivity: Species reactivity includes human; predicted by homology: mouse, rat, bovine, pig; others not known.
Product Description: Rabbit polyclonal Anti-Bax antibody is supplied as 0.25 ml of affinity purified rabbit IgG at 200 µg/ml in 1X PBS (pH 7.4) with 0.05% sodium azide.
Immunogen: Rabbit Anti-Bax polyclonal antibody was raised against a synthetic peptide derived from internal region of human Bax protein.
Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
IHC(Formalin/paraffin): 1:50 for 30 min at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temperature for 20 min.
Figure 1. IHC of normal human breast cells stained with rabbit Anti-BAX antibody (Cat# 53252).