Host: Rabbit Species Reactivity: Human; Predicted by homology: Bovine, Chicken, Mouse, Pig and Rat; Others not tested. Application: IHCProduct Description: Rabbit polyclonal Anti-SP1 antibody is supplied as 0.25 ml of affinity purified rabbit IgG at 200 µg/ml in 1X PBS (pH 7.4) with 0.05% sodium azide. Immunogen: Rabbit polyclonal Anti-SP1 antibody was raised against a synthetic peptide corresponding to the C-terminus region of human SP1.Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. WB: 1:25 IHC (Formalin/paraffin): 1:200 for 30 min at room temperature (staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10 min followed by cooling at room temperature for 20 min). Figure 1. IHC of human tonsil cells (formalin-fixed/ paraffin-embedded) stained with rabbit polyclonal Anti-SP1 antibody (Cat# 53855).