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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase  >>  SensoLyte® 520 TEV Activity Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 TEV Activity Assay Kit *Fluorimetric*  NEW
Size 1 kit
Catalog # AS-72227
US$ $357
Description

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal. The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL® 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL® 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

The kit contains: • 5-FAM/QXL® 520 TEV substrate, Ex/Em=490/520 nm upon cleavage• Assay buffer • Recombinant TEV Protease• DTT • Fluorescence reference standard for calibration • A detailed protocolKit size: 100 assays (96-well plate) Figure 1. Proteolytic cleavage of 5-FAM-QXL® 520 labeled TEV substrate. Fluorescence signal was measured with a fluorescence microplate reader (Flexstation 384II, Molecular Devices) at Ex/Em=490 nm/520 nm.

Images SensoLyte<sup>®</sup> 520 TEV Activity Assay Kit <i>*Fluorimetric*</i>
Detailed Information Datasheet
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