Login
Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Password:
Stay Logged In


Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase  >>  Sortase A protease, Recombinant

Product Name Sortase A protease, Recombinant  NEW
Size 10 µg
Catalog # 72229
US$ $374
Description

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Sortase A protease can be used with SensoLyte®520 Sortase A Activity Assay Kits (AnaSpec Cat# 72228) to screen for anti-infective agents.

Sortase A from Staphylcoccus aureus was expressed in E. coli expression system with a N-terminal His tag. It is comprised of 26-206 amino acids and its MW is 21.7-kDa. The activity of Sortase A can be measured in a FRET-based enzymatic assay, SensoLyte® 520 Sortase A Activity Assay Kit, Cat# 72228. 100 ng of enzyme is sufficient for the assay.

Sortase A is stored in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, and 2.2 mM KCl, 8 mM imidazole, 0.04% Tween 20, and 20 % glycerol.

The purity is >87% as estimated on SDS PAGE.

Related Product: SensoLyte® 520 Sortase A Activity Assay Kit *Fluorimetric*, Cat# 72228

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
     
  < Back