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Antibodies  >  Primary Antibodies  >  Chromatin and Nuclear Signaling  >>  Monoclonal Antibody against 5-Hydroxymethylcytosine (5-hmC)

Product Name Monoclonal Antibody against 5 - Hydroxymethylcytosine (5 - hmC)  NEW
5 - hmC Monoclonal Antibody
Size 100 g
Catalog # BI-HMCY-0100
US$ $499
Description

Host: Mouse

Clone: 4D9

Isotype: IgG1 Concentration: 1 mg/ml

Species Reactivity: All species

Specificity: 5-hmC (5-Hydroxymethylcytosine)

Epitope: Modified 5-hydroxymethylcytosine found in DNA of vertebrates

Purity: >95% (Purified IgG fractions prepared by affinity chromatography using Protein A)

Storage Buffer: Phosphate Buffer Saline 10 mM, 0.15M NaCl, pH 7.4

Storage Instructions: Store aliquoted at -20C. Avoid repeated freeze/thaw cycles

Validated Applications: These are suggested amounts/dilutions. Optimal condition should be determined by the end user.Hydroxymethyl DNA Immunoprecipitation: 1-3 µg per IPIF: 1:500ELISA: 1:1000Dot Blot: 1:2000

RELATED PRODUCTS:

Monoclonal Antibody against 5 - Methylcytosine (available in 3 sizes 0.1, 0.5 & 1 mg)

Starter Kit: 50 ug each of 5-Methylcytosine (5-mC) mAb, clone 33D3 and 5-hydroxymethylcytosine (5-hmC) mAb, clone 4D9

Figure 1. Dot blot of 5-hmC mAb (4D9) with DNA standard, Cytosine (C), 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC).

Figure 2. Titer of 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (4D9). Estimated titer is 0.05 µg/ml.
Images Monoclonal Antibody against 5-Hydroxymethylcytosine (5-hmC)
Storage -20C
     
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