Species Reactivity: Species reactivity includes human, mouse, rat, bovine and monkey, while others have not been tested. This antibody was evaluated for specificity with dot blot assay (using synthetic tau peptides) and Western blot (using recombinant human tau and mouse whole brain lysate).
Application: ELISA, dot blot, WB
Product Description: Rabbit polyclonal Anti-Tau Antibody (K280) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 µg/µl) 1x PBS (pH 7.5) containing BSA and 0.02% Sodium Azide.
Immunogen: Rabbit Anti-Tau (K280) polyclonal antibody was raised against a synthetic peptide corresponding to human Tau sequence surrounding Lysine 280.
Application Notes: The following are recommended concentration ranges. However, optimal working concentrations for specific application should be determined by the investigator.
Dot Blot: 1:200-2,000
Immunoprecipitation*: 3-5 µg/extract from 107 cells
*Recommended but not tested
Anti-Tau Antibody (Acetyl K280), Rabbit Polyclonal - Antibody raised against Tau acetylated K280. It recognizes acetylated Tau K280 only
Anti-Tau Antibody (GlcNAc Ser400), Rabbit Polyclonal - Antibody raised against Tau glycosylated Ser400
Anti-Tau Antibody (Ser400), Rabbit Polyclonal - Antibody raised against Tau Ser400. This antibody recognizes Ser400 modifications, including glycosylated and phosphorylated Ser400
Anti-Tau Antibodies - Wide selection of phospho & non-phospho specific 36+ Tau antibodies
Tau (441) Human Recombinant Proteins, with or without GST tag
Tau Peptides - 6 isoforms &[Ser(OGlcNAc)]400-KWK-Tau (388-411)-KK(Biotin)-NH2
Spotlight: Tau Products - Antibodies, Recombinant Proteins and Peptides
Figure 1. Dot blot of rabbit anti-Tau (K280) polyclonal antibody using synthetic peptides: Tau (Acetyl K280), Tau (K280) dotted at 0.02, 0.2 and 1 µg.
Figure 2. WB of mouse brain lysate and recombinant Tau proteins using rabbit anti-Tau (K280) polyclonal antibody. M: Marker. Lane 1: mouse brain lysate. Lane 2: Recombinant human Tau protein acetylated by p300 enzyme. Lane 3: Recombinant human Tau protein.