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Apoptosis, a programmed, cell-autonomous death process, plays important roles in a variety of physiological and pathological events.1 These range from normal fetal development to diseases, such as cancer2 organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase,3 DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C delta.4 At least ten caspases have been discovered. Some of these caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate.4
AnaSpec is pleased to showcase its line of SensoLyte® Caspase Assay Kits. With features that include one-step homogeneous assays, a convenient mix-and-read format, and highly sensitive fluorimetric components, SensoLyte® Caspase Assay Kits are an ideal solution for high-throughput screening.
References:
- Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
- Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
- Lazebnik, YA. et al. Nature 371, 346-347 (1994).
- Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).
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