Enzyme Detection Reagents

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Fluorescent Dyes > Enzyme Detection Reagents

Proteolytic cleavage of peptide bonds is one of the most important mechanisms affecting the properties and functions of proteins. Proteases are pervasively distributed in all tissues and biological fluids. They play essential roles in protein activation, cell regulation and signaling. They also play a role in the generation of amino acids for protein synthesis or for use in other metabolic pathways.

AnaSpec offers one of the world's most extensive collections of chromogenic and fluorogenic substrates for you to analyze your target proteases and inhibitors. These include selective protease substrates for caspases, MMPs, secretases and viral proteases such as HIV and HCV. Our EnzoLyte™ protease assay kits provide speed, convenience and accuracy for analyzing protease activities and screening protease inhibitors. To further address your specific needs in protease research, we offer competitive custom synthesis and assay design for any proteases that might interest you.

Product	Size	Catalog #	US$
(Z - AA)2Rh110 [Rhodamine 110, bis - (CBZ - L - alanyl - L - alanine amide)]	5 mg	60320-5	$115

(Z - Ala - Ala - Ala - Ala)2Rh110 [Rhodamine 110, bis - (CBZ - L - alanyl - L - alanyl - L - alanyl - L - alanine amide)]	5 mg	60321-5	$115

(Z - AR)2Rh110•2HCl [Rhodamine 110, bis - (CBZ - L - alanyl - L - arginine amide)]	5 mg	60322-5	$115

(Z - Arg)2Rh110 • 2HCl [Rhodamine 110, bis - (CBZ - L - arginine amide)]	5 mg	60323-5	$115

2',7' - Dichlorodihydrofluorescein diacetate H2DCFDA, H2DCF DA	100 mg	85706	$93

3 - Cyano - 7 - ethoxycoumarin	10 mg	85705	$66

360 MMP FRET Substrate I	1 mg	27074	$99

360 MMP FRET Substrate I	5 mg	27075	$341

360 MMP FRET Substrate II	1 mg	27082	$71

360 MMP FRET Substrate II	5 mg	27083	$291

360 MMP FRET Substrate III	1 mg	27094	$71

360 MMP FRET Substrate IV	1 mg	27098	$71

360 MMP FRET Substrate IV	5 mg	27099	$291

360 MMP FRET Substrate V	1 mg	27102	$71

360 MMP FRET Substrate VI	1 mg	27106	$71

360 MMP FRET Substrate VI	5 mg	27107	$291

390 MMP FRET Substrate I Mca - PLGL - Dpa - AR - NH2	1 mg	27076	$99

390 MMP FRET Substrate I Mca - PLGL - Dpa - AR - NH2	5 mg	27077	$396

390 MMP FRET Substrate II Mca - PLGL - Dpa - AR	5 mg	27079	$385

390 MMP FRET Substrate III Mca - PLA - Nva - Dpa - AR - NH2	1 mg	27090	$99

390 MMP FRET Substrate IV Mca - P - Cha - G - Nva - HA - Dpa - NH2	1 mg	27108	$99

390 MMP FRET Substrate IV Mca - P - Cha - G - Nva - HA - Dpa - NH2	5 mg	27109	$385

4 - CN [4 - Chloro - 1 - naphthol], 1M solution in DMSO	5 mL	85503	$66

4 - Methylumbelliferyl - β - D - glucoside	250 mg	85607	$61

4 - Nitrophenyl - α - D - galactopyranoside	1 g	85638	$66

4 - Nitrophenyl - N - acetyl - b - D - galactosaminide	100 mg	85618	$121

490 MMP FRET Substrate III	1 mg	27104	$220

490 MMP FRET Substrate IV	0.1 mg	60637	$93

490 MMP FRET Substrate V	1 mg	60563-1	$363

5 - (and - 6) - Carboxy - 2',7' - dichlorodihydrofluorescein diacetate	25 mg	85703	$93

5 - Carboxy - 2',7' - dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester)	5 mg	85702	$93

5 - FAM MMP FRET Peptide Fluorescence Standard I	0.1 mg	60584-01	$115

520 MMP FRET Substrate I	0.1 mg	60568-01	$159

520 MMP FRET Substrate II	0.1 mg	60569-01	$159

520 MMP FRET Substrate III	0.1 mg	60570-01	$159

520 MMP FRET Substrate IV	0.1 mg	60571-01	$159

520 MMP FRET Substrate IX	0.1 mg	60576-01	$159

520 MMP FRET Substrate V	0.1 mg	60572-01	$159

520 MMP FRET Substrate VI	0.1 mg	60573-01	$159

520 MMP FRET Substrate VII	0.1 mg	60574-01	$159

520 MMP FRET Substrate VIII	0.1 mg	60575-01	$159

520 MMP FRET Substrate X	0.1 mg	60577-01	$159

520 MMP FRET Substrate XI	0.1 mg	60578-01	$159

520 MMP FRET Substrate XII	0.1 mg	60579-01	$159

520 MMP FRET Substrate XIII	0.1 mg	60580-01	$159

520 MMP FRET Substrate XIV	0.1 mg	60581-01	$159

520 MMP FRET Substrate XV	0.1 mg	60582-01	$159

520 MMP FRET Substrate XVI	0.1 mg	60583-01	$159

520 MMP FRET Substrate XVII	0.1 mg	60554-01	$159

580 MMP FRET Substrate I	0.1 mg	60585-01	$159

7 - Ethoxy - 4 - trifluoromethylcoumarin	25 mg	85716	$66

Ac - IETD - AnaRed™	1 mg	60314-1	$215

ADHP 10 - Acetyl - 3,7 - dihydroxyphenoxazine	25 mg	85500	$247

Alpha - Secretase Substrate 1	1 mg	60271	$253

Benzyloxyresorufin [Resorufin benzyl ether]	10 mg	85726	$66

Beta - Secretase Substrate 1a	1 mg	60268	$253

Beta - Secretase Substrate 2	1 mg	60269	$253

Casein, FITC Conjugated	5 mg	85100	$61

Casein, TAMRA Conjugated	5 mg	85101	$121

Caspase 1 (ICE) Substrate 1m, fluorogenic Ac - YEVD - AMC	5 mg	25267-5	$154

Caspase 1 (ICE) Substrate 2, chromogenic Ac - YVAD - pNA	5 mg	25259-5	$105

Caspase 1 (ICE) Substrate 2f, fluorogenic Ac - YVAD - AFC	5 mg	25271-5	$181

Caspase 1 (ICE) Substrate 2f, fluorogenic Ac - YVAD - AFC	25 mg	25271-25	$726

Caspase 1 (ICE) Substrate 2m, fluorogenic Ac - YVAD - AMC	25 mg	25261-25	$605

Caspase 1 (ICE) Substrate 2m, fluorogenic Ac - YVAD - AMC	5 mg	25261-5	$154

Caspase 1 (ICE) Substrate 2r, fluorogenic (Ac - YVAD)2 - Rh110	1 mg	60310-1	$154

Caspase 1 (ICE) Substrate 3f, fluorogenic Ac - WEHD - AFC	5 mg	25285-5	$181

Caspase 1 (ICE) Substrate 3f, fluorogenic Ac - WEHD - AFC	25 mg	25285-25	$726

Caspase 1 (ICE) Substrate 3m, fluorogenic Ac - WEHD - AMC	25 mg	25275-25	$605

Caspase 2 (ICH - 1) Substrate 1m, fluorogenic Ac - VDVAD - AMC	5 mg	25255-5	$154

Caspase 3 (Apopain) Substrate 1, chromogenic Ac - DEVD - pNA	5 mg	25263-5	$83

Caspase 3 (Apopain) Substrate 1f, fluorogenic Ac - DEVD - AFC	5 mg	25273-5	$181

Caspase 3 (Apopain) Substrate 1f, fluorogenic Ac - DEVD - AFC	25 mg	25273-25	$726

Caspase 3 (Apopain) Substrate 1m, fluorogenic Ac - DEVD - AMC	25 mg	25262-25	$605

Caspase 3 (Apopain) Substrate 1m, fluorogenic Ac - DEVD - AMC	5 mg	25262-5	$154

Caspase 3 (Apopain) Substrate 1r - z, fluorogenic (Z - DEVD)2 - Rh110	5 mg	60304-5	$350

Caspase 6 (Mch2) Substrate 1f, fluorogenic Ac - VEID - AFC	5 mg	25272-5	$181

Caspase 6 (Mch2) Substrate 1m, fluorogenic Ac - VEID - AMC	5 mg	25256-5	$154

Caspase 6 (Mch2) Substrate 1r, fluorogenic (Ac - VEID)2 - Rh110	1 mg	60308-1	$154

Caspase 8 Substrate 1, chromogenic Ac - IETD - pNA	5 mg	25258-5	$105

Caspase 8 Substrate 1f, fluorogenic Ac - IETD - AFC	5 mg	25270-5	$181

Caspase 8 Substrate 1f, fluorogenic Ac - IETD - AFC	25 mg	25270-25	$726

Caspase 8 Substrate 1f - z, fluorogenic Z - IETD - AFC	5 mg	60324-5	$154

Caspase 8 Substrate 1m, fluorogenic Ac - IETD - AMC	5 mg	25257-5	$154

Caspase 8 Substrate 1m, fluorogenic Ac - IETD - AMC	25 mg	25257-25	$605

Caspase 8 Substrate 1r - z, fluorogenic (Z - IEHD)2 - Rh110	5 mg	60312-5	$237

Caspase 8 Substrate 1r - z, fluorogenic (Z - IETD)2 - Rh110	1 mg	60312-1	$154

Caspase 9 Substrate 1, chromogenic Ac - LEHD - pNA	5 mg	25278-5	$105

Caspase 9 Substrate 1f, fluorogenic Ac - LEHD - AFC	5 mg	25276-5	$181

Caspase 9 Substrate 1f, fluorogenic Ac - LEHD - AFC	25 mg	25276-25	$726

Caspase 9 Substrate 1r, fluorogenic (Ac - LEHD)2 - Rh110	5 mg	60306-5	$215

Caspase 9 Substrate 1r, fluorogenic (Ac - LEHD)2 - Rh110	1 mg	60306-1	$154

Caspase 9 Substrate 2m, fluorogenic Ac - LEHD - AMC	5 mg	25286-5	$181

Caspase 9 Substrate 2m, fluorogenic Ac - LEHD - AMC	25 mg	25286-25	$726

Collagen (Type I), FITC conjugated Water Insoluble	5 mg	85102	$61

Collagen (Type I), FITC conjugated Water Soluble	1 mg	85111	$247

Collagen (Type IV), FAM conjugated	1 mg	85112	$215

D - Luciferin, free acid UltraPure Grade	25 mg	82250	$83

D - Luciferin, potassium salt UltraPure Grade	25 mg	82252	$105

D - Luciferin, sodium salt	25 mg	82251	$93

Dihydroethidium [Hydroethidine], 5 mM solution in DMSO	1 mL	85718	$154

Dihydroethidium [Hydroethidine] Special Air - free Packaging	10 mg	85717	$193

Dihydrofluorescein diacetate [H2FDA]	25 mg	85710	$66

Dihydrorhodamine 123	10 mg	85711	$93

Dihydrorhodamine 123 Air - free Packaging	10 mg	85720	$159

Dihydrorhodamine 123, 5 mM solution in DMSO	1 mL	85719	$154

Dihydrorhodamine 6G	25 mg	85712	$93

Elastin, FITC Conjugated	1 mg	85113	$121

Elastin, FITC - Conjugated Water Insoluble	1 mg	85103	$121

Ethoxyresorufin [Resorufin ethyl ether]	5 mg	85715	$66

FDG [Fluorescein di - β - D - galactopyranoside]	5 mg	85600	$121

Gelatin, FITC Conjugated	5 mg	85145	$61

HCV Protease FRET Substrate (RET S1)	0.25 mg	22991-025	$181

HCV Protease FRET Substrate (RET S1)	1 mg	22991	$583

HIV Protease FRET Substrate I	1 mg	22992	$231

HPPA [3 - (4 - Hydroxyphenyl)propionic acid]UltraPure Grade	1 g	85505	$93

Malaria Aspartyl Proteinase FRET Substrate I [DABCYL - Glu - Arg - Nle - Phe - Leu - Ser - Phe - Pro - EDANS]	1 mg	24480	$220

Malaria Aspartyl Proteinase FRET Substrate I [DABCYL - Glu - Arg - Nle - Phe - Leu - Ser - Phe - Pro - EDANS]	5 mg	24481	$869

Mca MMP FRET Peptide Fluorescence Standard I	1 mg	27159	$39

Mca MMP FRET Peptide Fluorescence Standard II	1 mg	27112	$61

Mca MMP FRET Peptide Fluorescence Standard II	5 mg	27113	$242

Methoxyresorufin [Resorufin methyl ether]	5 mg	85707	$66

MMP Colorimetric Substrate I	1 mg	27096	$121

MMP Colorimetric Substrate I	5 mg	27097	$484

MMP Peptide Inhibitor I	1 mg	27157	$49

MMP Peptide Inhibitor I	5 mg	27158	$193

MPTS [8 - Methoxypyrene - 1,3,6 - trisulfonic acid, trisodium salt]	100 mg	85725	$66

MUGlcU [4 - Methylumbelliferyl - β - D - glucuronide]	100 mg	85606	$66

MUP [4 - Methylumbelliferyl phosphate, free acid] UltraPure Grade	1 g	85310	$93

MUP, DCA [4 - Methylumbelliferyl phosphate, dicyclohexylammonium salt, trihydrate] UltraPure Grade	1 g	85311	$93

NFF - 2	5 mg	27111	$385

NFF - 3	1 mg	27114	$99

NFF - 3	5 mg	27115	$385

Pentoxyresorufin [Resorufin pentyl ether]	5 mg	85728	$66

pNPP [4 - Nitrophenyl phosphate, disodium salt] UltraPure Grade	1 g	85301	$121

Renin FRET Substrate I (DABCYL - g - Abu - Ile - His - Pro - Phe - His - Leu - Val - Ile - His - Thr - EDANS)	1 mg	24478	$220

Resazurin, sodium salt UltraPure Grade	100 mg	85740	$66

Resorufin β - D - Galactopyranoside	25 mg	85617	$93

TACE FRET Substrate I (DABCYL - Leu - Ala - Gln - Ala - Val - Arg - Ser - Ser - Ser - Arg - EDANS)	5 mg	25349	$946

TACE FRET Substrate I (DABCYL - Leu - Ala - Gln - Ala - Val - Arg - Ser - Ser - Ser - Arg - EDANS)	1 mg	25043	$237

TNO113	1 mg	27061	$291

TNO211	1 mg	25350	$253

TNO211	5 mg	25350-5	$1017

WAAG - 3R, Aggrecanase (ADAM - TS - 4) FRET Substrate Abz - TEGEARGSVI - Dpa - KK - NH2	1 mg	60431-1	$237

X - Gal [5 - Bromo - 4 - chloro - 3 - indolyl - β - D - galactopyranoside]	1 g	85620	$121

X - GlcU [5 - Bromo - 4 - chloro - 3 - indoxyl - β - D - glucuronic acid, sodium salt]	25 mg	85630	$121

X - GluNAc [5 - Bromo - 4 - chloro - 3 - indoxyl - N - acetyl - β - D - glucosaminide]	25 mg	85623	$121

Z - DEVD - AFC	5 mg	21685-5	$181

Z - DEVD - AFC	25 mg	21685-25	$726

Z - DEVD - AMC Z - DEVD - AMC	5 mg	60303-5	$181

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