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| Secretase Research Solutions - FRET Secretase Substrates, Assay Kits & Inhibitors |
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Secretase Research Solutions
As scientific research explores the role of ß-Amyloid in the onset of Alzheimer’s, the secretases whose cleavage activity produces ß-Amyloid fragments have become an important focus. As the world’s most comprehensive provider of ß-amyloid peptides, AnaSpec continues to expand its product solutions to meet the growing needs of the Alzheimer’s research community. We are pleased to introduce a new line of amyloid-related secretase substrates, assay kits and inhibitors.
Amyloid Precursor Protein, APP, a transmembrane glycoprotein of about 770 amino acids, is cleaved by α-Secretase and ß-Secretase to produce C-terminal fragments that are 83 amino acids (C83) and 99-amino acids (C99) in length, respectively. Subsequent cleavages of C83 and C99 by γ-Secretase produce a 3-kD (p3) protein in the former and a 4-kD (ß-amyloid) protein in the latter and a CT57-59 in both fragments (reviewed in 1, 2). The 4-Kd consists of Aß which are 39 to 42 amino acids in length, with Aß 1-42 being the major component of amyloid plaques which accumulates in neurons of Alzheimer’s disease (AD) brain (1, 2). Consequently, ß-Secretase is an important target for AD drug development.
FRET Secretase Substrates
AnaSpec provides fluorescence resonance energy transfer (FRET) substrates for use in measuring ß-Secretase activity. Upon cleavage of the FRET substrate by ß-Secretase, the quenched fluorescence of the donor is released, with the fluorescence signal increasing with ß-Secretase activity. Variations include a range of sequence lengths and emission wavelengths.
Wild-Type
Swedish Double Mutation (K670N/M671L)
EnzoLyte™ 520 ß-Secretase Assay Kit
Ready-to-use assay kit for detection of ß-Secretase V. 100 assays.
ß-Secretase inhibitors
High-purity peptides that inhibit ß-Secretase activity.
a -Secretase Substrate
A FRET-based a-Secretase substrate, which upon cleavage liberates the Mca (7-Methoxycoumarin-4-acetic acid) fragment from the proximity quenching effect of Dnp (2,4-dinitrophenyl), Abs/Em=325/393 nm.
To request AnaSpec’s 2006-2008 Peptides or 2006-2007 Detection Reagents & Kits Catalogs, click here.
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