SensoLyte® 520 Generic Caspase Assay and Other Apoptosis Kits, Peptides & Antibodies


AnaSpec is pleased to introduce the newest member of the Caspase Kits series – the SensoLyte® 520 Generic Caspase Assay Kit, and showcase our other apoptosis product offering. This includes a suite of SensoLyte® Caspase and Cytotoxicity Assay Kits, caspase and apoptosis related peptides, and antibodies, including zebrafish specific antibodies.

SensoLyte® 520 Generic Caspase Assay Kit * Fluorimetric * - reagent is cell permeable
and non-cytotoxic

SensoLyte® Caspase Assay Kits * Fluorimetric * - Emission wavelength across the
spectrum

SensoLyte® Cytotoxicity & Viability Assay Kits * Fluorimetric *

Caspase and Apoptosis Related Peptides

Apoptosis & Autophagy Antibodies - Z-Fish™ & Other Species Specific

SensoLyte® 520 Generic Caspase Assay Kit NEW

This kit is ideal for in situ detection of caspase activity in cells undergoing apoptosis using a fluorochrome inhibitor of caspase, FAM-VAD-FMK. This reagent is cell permeable and non-cytotoxic. When added to a population of cells, it enters cells and covalently binds to activated caspases-1, -3, -4, -5, -7, -8, and –9. Bound FAM-VAD-FMK is retained within the cell, while any unbound FAM-VAD-FMK diffuses out of the cells and is washed away. Signal in the cells containing the bound FAM-VAD-FMK can be evaluated with a fluorescence plate reader, fluorescence microscopy, or flow cytometry.

Caspase Assay Kit * Fluorimetric *

#Assays(96-well plate)

Catalog #

SensoLyte® 520 Generic Caspase Assay Kit NEW
Ex/Em = 490/520 nm

100 assays

72211




Figure 1. Active caspase was detected with FAM-VAD-FMK in Jurkat cells (triplicates). Apoptosis was induced with 1mM staurosporin for 4 h and control cells were treated with DMSO at 37°C under 5% CO2. After treatment, FAM-VAD-FMK was added at 37°C for 1 h. Fluorescence signal was measured by a microplate reader (FlexStation II384, Molecular Device, CA) at Ex/Em=490 nm/520 nm.

SensoLyte® Caspase Assay Kits

The SensoLyte® Caspase Assay Kits are one-step homogeneous assays. Designed in a convenient mix-and-read format, and containing highly sensitive fluorimetric components, SensoLyte® Caspase Assay Kits are an ideal solution for high-throughput screening.

Caspase Assay Kits * Fluorimetric *

#Assays

(96-well plate)

Catalog #

SensoLyte® AMC Caspase Profiling Kit (8 pre-coated caspase substrates)
Ex/Em = 354/442 nm

Two-96 well plates

71120

SensoLyte® AMC Caspase Substrate Sampler Kit (8 caspase substrates)
Ex/Em = 354/442 nm

8 x 100 assays

71121

SensoLyte® AFC Caspase Profiling Kit (8 pre-coated caspase substrates)
Ex/Em = 380/500 nm

Two-96 well plates

71116

SensoLyte® AFC Caspase Substrate Sampler Kit (8 caspase substrates)
Ex/Em = 380/500 nm

8 x 100 assays

71117

SensoLyte® Homogeneous AMC Caspase - 3/7 Assay Kit
Ex/Em = 354/442 nm

500 assays

71118

SensoLyte® Homogeneous AFC Caspase - 3/7 Assay Kit
Ex/Em = 380/500 nm

500 assays

71114

SensoLyte® Homogeneous Rh110 Caspase - 3/7 Assay Kit
Ex/Em = 496/520 nm

500 assays

71141

SensoLyte® Homogeneous AnaRed Caspase - 3/7 Assay Kit
Ex/Em = 537/611 nm

500 assays

71122

SensoLyte® Homogeneous AFC Caspase - 8 Assay Kit
Ex/Em = 380/500 nm

100 assays
200 assays

72088-100
72088-200


SensoLyte® Cytotoxicity & Viability Assay Kits

Cytotoxicity / Cell Viability / Proliferation Kits * Fluorimetric *

#Assays
(96-well plate)

Catalog #

Sensolyte® Cell Cytotoxicity Assay Kit
Ex/Em = 560/590 nm

500 assays
5,000 assays

71302
71303

Sensolyte® Cell Viability and Proliferation Assay Kit
Ex/Em = 560/590 nm

2,000 assays
10,000 assays

71300
71301

SensoLyte® Calcein Cell Viability Assay Kit
Ex/Em = 494/520 nm

1,000 assays

72126

Caspase and Apoptosis Related Peptides

Substrate

Inhibitor

pNA

Abs=408 nm

AFC

Ex/Em=370/500 nm

AMC

Ex/Em=340/440 nm

Rh 110

Ex/Em=501/527 nm

CHO

Ac-DEVD

25263-5
(5 mg)

25263-25
(25 mg)

25273-5
(5 mg)

25273-25
(25 mg)

25262-5
(5 mg)

25262-25
(25 mg)

25269-5
(5 mg)

25269-25
(25 mg)

Z-DEVD

25282-5
(5 mg)

25282-25
(25 mg)

21685-5
(5 mg)

25685-25
(25 mg)

60303-5
(5 mg)

60304-5
(5 mg)

Ac-DMQD

25260-5
(5 mg)

25260-25
(25 mg)

60325-5
(5 mg)

25266-5
(5 mg)

25266-25
(25 mg)

25279-5
(5 mg)

25279-25
(25 mg)

Ac-DQMD

25265-5
(5 mg)

25265-25
(25 mg)

Ac-IETD

25258-5
(5 mg)

25258-25
(25 mg)

25270-5
(5 mg)

25270-25
(25 mg)

25257-5
(5 mg)

25257-25
(25 mg)

25274-5
(5 mg)

25274-25
(25 mg)

Z-IETD

60324-5
(5 mg)

60312-5
(5 mg)

Ac-LEHD

25278-5
(5 mg)

25278-25
(25 mg)

25276-5
(5 mg)

25276-25
(25 mg)

25286-5
(5 mg)

25286-25
(25 mg)

60306-5
(5 mg)

23590
(5 mg)

Ac-VDQQD

25251-5
(5 mg)

25251-25
(25 mg)

Ac-VDVAD

25252-5
(5 mg)

25252-25
(25 mg)

25264-5
(5 mg)

25264-25
(25 mg)

25255-5
(5 mg)

25255-25
(25 mg)

61203
(5 mg)

Ac-VQVD

25253-5
(5 mg)

25253-25
(25 mg)

Ac-WEHD

60843
(1 mg)

25285-5
(5 mg)

25285-25
(25 mg)

25275-5
(5 mg)

25275-25
(25 mg)

60309-1
(1 mg)

60863
(1 mg)

Ac-WVAD

60857
(1 mg)

Ac-YEVD

25250-5
(5 mg)

25250-25
(25 mg)

25267-5
(5 mg)

25267-25
(25 mg)

Ac-YVAD

25259-5
(5 mg)

25259-25
(25 mg)

25271-5
(5 mg)

25271-25
(25 mg)

25261-5
(5 mg)

25261-25
(25 mg)

60310-1
(1 mg)

25277-5
(5 mg)

25277-25
(25 mg)

Apoptosis Antibodies - Z-Fish™ Specific

Product

Size

Catalog #

Anti - Bad (IN), Z - Fish™

100 uL
250 uL

55478s
55478

Anti - Bax - a (NT), Z - Fish™

100 uL
250 uL

55469s
55469

Anti - Bax - b (NT), Z - Fish™

100 uL
250 uL

55498s
55498

Anti - Bbc - 3 (IN), Z - Fish™
p53 upregulated modulator of apoptosis; BCL2 binding component 3 (bbc3)

250 uL

55548

Anti - Bcl - 2 (NT), Z - Fish™

100 uL

55396s
55396

Anti - Bcl - xl (IN), Z - Fish™

100 uL

55426s
55426

Anti - Bcl2l10 (NT), Z - Fish™

100 uL
250 uL

55467s
55467

Anti - Caspase 8l2 (IN), Z - Fish™
Caspase 8 like 2

250 uL

55376

Anti - Caspase - 2 (IN), Z - Fish™

100 uL
250 uL

55368s
55368

Anti - Caspase - 2 (NT), Z - Fish™

100 uL
250 uL

55367s
55367

Anti - Caspase - 3a (p12) (CT), Z - Fish™

100 uL
250 uL

55372s
55372

Anti - Caspase - 3a (p17) (NT), Z - Fish™

100 uL
250 uL

55371s
55371

Anti - Caspase - 3b (p12) (CT), Z - Fish™

100 uL
250 uL

55374s
55374

Anti - Caspase - 3b (p17) (NT), Z - Fish™

250 uL

55373

Anti - Caspase - 8 (IN), Z - Fish™

100 uL
250 uL

55375s
55375

Anti - Caspase - 9 (IN - 3) (p10), Z - Fish™

100 uL
250 uL

55379s
55379

Anti - Caspase - 9 (p37) (IN - 1), Z - Fish™

100 uL
250 uL

55377s
55377

Anti - Caspase - 9 (p37) (IN - 2), Z - Fish™

100 uL
250 uL

55378s
55378

Anti - Caspase - a (IN), Z - Fish™

100 uL
250 uL

55369s
55369

Anti - Caspase - b (IN), Z - Fish™

100 uL
250 uL

55370s
55370

Anti - Cflar (IN), Z - Fish™
CASP8 and FADD - like apoptosis regulator; FLIP

250 uL

55505

Apoptosis Antibodies –Other Species

Product

Size

Catalog #

Anti - Caspase - 1 (CT)
ICE, IL - 1b converting enzyme

50 µg

54209

Anti - Caspase - 1 (IN)
ICE, IL - 1b converting enzyme

50 µg

54210

Anti - Caspase - 3 (p12)

50 µg

55040

Anti - Caspase - 3 (p17)

50 µg

55041

Anti - Caspase - 4 (NT)
ICH - 2, ICErelII, Mih1

50 µg

54206

Anti - Caspase - 5 (IN)
ICH - 3, ICErelIII

50 µg

53296

Anti - Caspase - 6 (NT)
Mch2

50 µg

53297

Anti - Caspase - 7 (CT)
ICELAP3, Mch3

50 µg

54211

Anti - Caspase - 7 (NT)
ICELAP3, Mch3

50 µg

54212

Anti - Caspase - 8 (IN)
FLICE, MACH, Mch5

50 µg

54215

Anti - Caspase - 8L

50 µg

29625

Anti - Caspase - 8

50 µg

53299

Anti - Caspase - 9

50 µg

53300

References:

1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).