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SensoLyte^®520 Generic Caspase Assay and Other Apoptosis Kits, Peptides & Antibodies

AnaSpec is pleased to introduce the newest member of the Caspase Kits series – the SensoLyte^® 520 Generic Caspase Assay Kit, and showcase our other apoptosis product offering. This includes a suite of SensoLyte^®Caspase and Cytotoxicity Assay Kits, caspase and apoptosis related peptides, and antibodies, including zebrafish specific antibodies.

• SensoLyte^® 520 Generic Caspase Assay Kit * Fluorimetric * - reagent is cell permeable
and non-cytotoxic

• SensoLyte^® Caspase Assay Kits * Fluorimetric * - Emission wavelength across the
spectrum

• SensoLyte^® Cytotoxicity & Viability Assay Kits * Fluorimetric *

• Caspase and Apoptosis Related Peptides

• Apoptosis & Autophagy Antibodies - Z-Fish™ & Other Species Specific

SensoLyte^® 520 Generic Caspase Assay Kit NEW

This kit is ideal for in situ detection of caspase activity in cells undergoing apoptosis using a fluorochrome inhibitor of caspase, FAM-VAD-FMK. This reagent is cell permeable and non-cytotoxic. When added to a population of cells, it enters cells and covalently binds to activated caspases-1, -3, -4, -5, -7, -8, and –9. Bound FAM-VAD-FMK is retained within the cell, while any unbound FAM-VAD-FMK diffuses out of the cells and is washed away. Signal in the cells containing the bound FAM-VAD-FMK can be evaluated with a fluorescence plate reader, fluorescence microscopy, or flow cytometry.

Caspase Assay Kit * Fluorimetric *	#Assays(96-well plate)	Catalog #
SensoLyte^® 520 Generic Caspase Assay Kit NEW Ex/Em = 490/520 nm	100 assays	72211

Figure 1. Active caspase was detected with FAM-VAD-FMK in Jurkat cells (triplicates). Apoptosis was induced with 1mM staurosporin for 4 h and control cells were treated with DMSO at 37°C under 5% CO₂. After treatment, FAM-VAD-FMK was added at 37°C for 1 h. Fluorescence signal was measured by a microplate reader (FlexStation II384, Molecular Device, CA) at Ex/Em=490 nm/520 nm.

SensoLyte^® Caspase Assay Kits

The SensoLyte^® Caspase Assay Kits are one-step homogeneous assays. Designed in a convenient mix-and-read format, and containing highly sensitive fluorimetric components, SensoLyte^® Caspase Assay Kits are an ideal solution for high-throughput screening.

Caspase Assay Kits * Fluorimetric *	#Assays (96-well plate)	Catalog #
SensoLyte^® AMC Caspase Profiling Kit (8 pre-coated caspase substrates) Ex/Em = 354/442 nm	Two-96 well plates	71120
SensoLyte^® AMC Caspase Substrate Sampler Kit (8 caspase substrates) Ex/Em = 354/442 nm	8 x 100 assays	71121
SensoLyte^® AFC Caspase Profiling Kit (8 pre-coated caspase substrates) Ex/Em = 380/500 nm	Two-96 well plates	71116
SensoLyte^® AFC Caspase Substrate Sampler Kit (8 caspase substrates) Ex/Em = 380/500 nm	8 x 100 assays	71117

SensoLyte^® Homogeneous AMC Caspase - 3/7 Assay Kit Ex/Em = 354/442 nm	500 assays	71118
SensoLyte^® Homogeneous AFC Caspase - 3/7 Assay Kit Ex/Em = 380/500 nm	500 assays	71114
SensoLyte^® Homogeneous Rh110 Caspase - 3/7 Assay Kit Ex/Em = 496/520 nm	500 assays	71141
SensoLyte^® Homogeneous AnaRed Caspase - 3/7 Assay Kit Ex/Em = 537/611 nm	500 assays	71122
SensoLyte^® Homogeneous AFC Caspase - 8 Assay Kit Ex/Em = 380/500 nm	100 assays 200 assays	72088-100 72088-200


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SensoLyte^® Cytotoxicity & Viability Assay Kits

Cytotoxicity / Cell Viability / Proliferation Kits * Fluorimetric *	#Assays (96-well plate)	Catalog #
Sensolyte^® Cell Cytotoxicity Assay Kit Ex/Em = 560/590 nm	500 assays 5,000 assays	71302 71303
Sensolyte^® Cell Viability and Proliferation Assay Kit Ex/Em = 560/590 nm	2,000 assays 10,000 assays	71300 71301
SensoLyte^® Calcein Cell Viability Assay Kit Ex/Em = 494/520 nm	1,000 assays	72126


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Caspase and Apoptosis Related Peptides

	Substrate				Inhibitor
	pNA Abs=408 nm	AFC Ex/Em=370/500 nm	AMC Ex/Em=340/440 nm	Rh 110 Ex/Em=501/527 nm	CHO
Ac-DEVD	25263-5 (5 mg) 25263-25 (25 mg)	25273-5 (5 mg) 25273-25 (25 mg)	25262-5 (5 mg) 25262-25 (25 mg)		25269-5 (5 mg) 25269-25 (25 mg)
Z-DEVD	25282-5 (5 mg) 25282-25 (25 mg)	21685-5 (5 mg) 25685-25 (25 mg)	60303-5 (5 mg)	60304-5 (5 mg)
Ac-DMQD	25260-5 (5 mg) 25260-25 (25 mg)	60325-5 (5 mg)	25266-5 (5 mg) 25266-25 (25 mg)		25279-5 (5 mg) 25279-25 (25 mg)
Ac-DQMD	25265-5 (5 mg) 25265-25 (25 mg)
Ac-IETD	25258-5 (5 mg) 25258-25 (25 mg)	25270-5 (5 mg) 25270-25 (25 mg)	25257-5 (5 mg) 25257-25 (25 mg)		25274-5 (5 mg) 25274-25 (25 mg)
Z-IETD		60324-5 (5 mg)		60312-5 (5 mg)
Ac-LEHD	25278-5 (5 mg) 25278-25 (25 mg)	25276-5 (5 mg) 25276-25 (25 mg)	25286-5 (5 mg) 25286-25 (25 mg)	60306-5 (5 mg)	23590 (5 mg)
Ac-VDQQD	25251-5 (5 mg) 25251-25 (25 mg)
Ac-VDVAD	25252-5 (5 mg) 25252-25 (25 mg)	25264-5 (5 mg) 25264-25 (25 mg)	25255-5 (5 mg) 25255-25 (25 mg)		61203 (5 mg)
Ac-VQVD	25253-5 (5 mg) 25253-25 (25 mg)
Ac-WEHD	60843 (1 mg)	25285-5 (5 mg) 25285-25 (25 mg)	25275-5 (5 mg) 25275-25 (25 mg)	60309-1 (1 mg)	60863 (1 mg)
Ac-WVAD			60857 (1 mg)
Ac-YEVD	25250-5 (5 mg) 25250-25 (25 mg)		25267-5 (5 mg) 25267-25 (25 mg)
Ac-YVAD	25259-5 (5 mg) 25259-25 (25 mg)	25271-5 (5 mg) 25271-25 (25 mg)	25261-5 (5 mg) 25261-25 (25 mg)	60310-1 (1 mg)	25277-5 (5 mg) 25277-25 (25 mg)


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Apoptosis Antibodies - Z-Fish™ Specific

Product	Size	Catalog #
Anti - Bad (IN), Z - Fish™	100 uL 250 uL	55478s 55478
Anti - Bax - a (NT), Z - Fish™	100 uL 250 uL	55469s 55469
Anti - Bax - b (NT), Z - Fish™	100 uL 250 uL	55498s 55498
Anti - Bbc - 3 (IN), Z - Fish™ p53 upregulated modulator of apoptosis; BCL2 binding component 3 (bbc3)	250 uL	55548
Anti - Bcl - 2 (NT), Z - Fish™	100 uL	55396s 55396
Anti - Bcl - 2 (NT), Z - Fish™
Anti - Bcl - xl (IN), Z - Fish™	100 uL	55426s 55426
Anti - Bcl2l10 (NT), Z - Fish™	100 uL 250 uL	55467s 55467
Anti - Caspase 8l2 (IN), Z - Fish™ Caspase 8 like 2	250 uL	55376
Anti - Caspase - 2 (IN), Z - Fish™	100 uL 250 uL	55368s 55368
Anti - Caspase - 2 (NT), Z - Fish™	100 uL 250 uL	55367s 55367
Anti - Caspase - 3a (p12) (CT), Z - Fish™	100 uL 250 uL	55372s 55372
Anti - Caspase - 3a (p17) (NT), Z - Fish™	100 uL 250 uL	55371s 55371
Anti - Caspase - 3b (p12) (CT), Z - Fish™	100 uL 250 uL	55374s 55374
Anti - Caspase - 3b (p17) (NT), Z - Fish™	250 uL	55373
Anti - Caspase - 8 (IN), Z - Fish™	100 uL 250 uL	55375s 55375
Anti - Caspase - 9 (IN - 3) (p10), Z - Fish™	100 uL 250 uL	55379s 55379
Anti - Caspase - 9 (p37) (IN - 1), Z - Fish™	100 uL 250 uL	55377s 55377
Anti - Caspase - 9 (p37) (IN - 2), Z - Fish™	100 uL 250 uL	55378s 55378
Anti - Caspase - a (IN), Z - Fish™	100 uL 250 uL	55369s 55369
Anti - Caspase - b (IN), Z - Fish™	100 uL 250 uL	55370s 55370
Anti - Cflar (IN), Z - Fish™ CASP8 and FADD - like apoptosis regulator; FLIP	250 uL	55505


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Apoptosis Antibodies –Other Species

Product	Size	Catalog #
Anti - Caspase - 1 (CT) ICE, IL - 1b converting enzyme	50 µg	54209
Anti - Caspase - 1 (IN) ICE, IL - 1b converting enzyme	50 µg	54210
Anti - Caspase - 3 (p12)	50 µg	55040
Anti - Caspase - 3 (p17)	50 µg	55041
Anti - Caspase - 4 (NT) ICH - 2, ICErelII, Mih1	50 µg	54206
Anti - Caspase - 5 (IN) ICH - 3, ICErelIII	50 µg	53296
Anti - Caspase - 6 (NT) Mch2	50 µg	53297
Anti - Caspase - 7 (CT) ICELAP3, Mch3	50 µg	54211
Anti - Caspase - 7 (NT) ICELAP3, Mch3	50 µg	54212
Anti - Caspase - 8 (IN) FLICE, MACH, Mch5	50 µg	54215
Anti - Caspase - 8L	50 µg	29625
Anti - Caspase - 8	50 µg	53299
Anti - Caspase - 9	50 µg	53300


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References:

1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).