SYBR® qPCR MasterMixes and Core Kits – Wide Selection


AnaSpec, EGT Group is pleased to offer a wide selection of SYBR® Green-based MasterMixes and Core Kits for almost any thermocycler available in the industry. Our MESA (Maximize & Enhance your SYBR® Assays) MasterMixes (see Tables 1-3) use our new powerful MeteorTaq hotstart polymerase.

Our MESA BLUE kits contain a proprietary inert BLUE dye that aids in the visualization of reagent dispensed, thus avoiding aberrant results before they occur. Our CORE kits are unique in the industry in that they are probably the only format in the industry that provide users with the ability to titrate SYBR® Green amount as part of the optimization process.

AnaSpec, EGT Group’s qPCR MasterMixes:

Reduce run times with uncompromised results
• Tailored for highly sensitive assays
• Optimized for maximum consistency and specificity
• Large dynamic range

Available in BLUE format
• New proprietary inert BLUE dye
• Clear visual dispensing of the reagent

To learn more about qPCR, please download the qPCR Guide by clicking here.

Figure 1. Comparison between MESA GREEN qPCR MasterMix Plus for SYBR® Assay Low ROX and Competitor S SYBR® Green kit. Experiment was performed using 18S primers and the same cDNA on a Mx3000P®. MESA GREEN MasterMix showed better sensitivity, earlier Cq (Ct) values, higher ΔRn and steeper amplification curves compared to Competitor S SYBR® Green reagent.

Table 1. MESA FAST MasterMix Plus for SYBR® assay uses our new powerful MeteorTaq hotstart polymerase which in combination with a newly formulated buffer results in greater sensitivity. Kits are optimized for use in fast cycling mode but can also be used in regular cycling mode.

Product

Size

Catalog #

MESA FAST qPCR MasterMix Plus for SYBR® Assay No ROX

- compatible for use in FAST cycling conditions on LC480 and Smartcyclers and RotorGene 6000.

600 RXNs

RT-SY2X-03+NRWOUF

MESA FAST qPCR MasterMix Plus dTTP for SYBR® Assay No ROX

- compatible for use in FAST cycling conditions on LC480, Smartcyclers and RotorGenes 6000/Q, and in regular cycling conditions on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, RotorGene 2000/3000/6000/Q, CFX96/384, DNA Engine Opticon® 1, Opticon® 2, Chromo 4, Mini Opticon®, Quantica® and LC480.

600 RXNs

RT-SY2X-03+NRWOUNF

MESA FAST qPCR MasterMix Plus for SYBR® Assay Low ROX

- compatible for use in FAST cycling conditions on ABI Prism® 7500.

600 RXNs

RT-SY2X-03+WOULRF

MESA FAST qPCR MasterMix Plus for SYBR® Assay ROX

- compatible for use in FAST cycling conditions on ABI Prism® 7900, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SY2X-03+WOUF

Table 2. MESA Green qPCR MasterMix Plus also uses our powerful MeteorTaq hotstart polymerase for greater sensitivity. Kits are optimized for use on regular cycling mode but can also be used in fast cycling mode.

Product

Size

Catalog #

MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX

- compatible for use on Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480.

600 RXNs

1200 RXNs

4000 RXNs

RT-SY2X-03+NRWOU

RT-SY2X-06+NRWOU

RT-SY2X-20+NRWOU

MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX + fluorescein

- fluorescein is needed to normalize the data correctly when using iCycler iQ®, iQ5 or My iQ® thermocyclers. This kit can be used on these machines.

600 RXNs

1200 RXNs

4000 RXNs

5 x 600 RXNs

RT-SY2X-03+WOUFL

RT-SY2X-06+WOUFL

RT-SY2X-20+WOUFL

05-SY2X-03+WOUFL

MESA GREEN qPCR MasterMix Plus for SYBR® Assay Low ROX

- compatible for use on ABI 7500, Mx4000®, Mx3000P® and Mx3005P®.

600 RXNs

1200 RXNs

4000 RXNs

RT-SY2X-03+WOULR

RT-SY2X-06+WOULR

RT-SY2X-20+WOULR

MESA GREEN qPCR MasterMix Plus dTTP for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SY2X-03+WOUN

MESA GREEN qPCR MasterMix Plus for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

1200 RXNs

4000 RXNs

RT-SY2X-03+WOU

RT-SY2X-06+WOU

RT-SY2X-20+WOU

One step MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX

- compatible for use on Mx4000®, Mx3000P®, Mx3005P®, DNA Engine Opticon® 1 & 2, Chromo 4, CFX96, Mini Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480.

600 RXNs

RT-SYRT-032XNR

One step MESA GREEN qPCR MasterMix Plus for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SYRT-032X

Table 3. MESA BLUE qPCR MasterMix Plus for SYBR® Assay uses our new powerful MeteorTaq hotstart polymerase in combination with a newly formulated buffer. A new proprietary inert Blue dye for visualization purpose that does not affect reaction is also included. These result in greater sensitivity and ease of use. Kits are optimized for use on fast cycling mode but can also be used in regular cycling mode.

Product

Size

Catalog #

MESA BLUE qPCR MasterMix Plus for SYBR® Assay No ROX

- compatible for use on Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480.

600 RXNs

RT-SY2X-03+NRWOUB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay No ROX + fluorescein

-fluorescein is needed to normalize the data correctly when using iCycler iQ®, iQ5 or My iQ® thermocyclers. This kit can be used on these machines.

600 RXNs

RT-SY2X-03+WOUFLB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay, Low ROX

- compatible for use on ABI 7500, Mx4000®, Mx3000P® and Mx3005P®.

600 RXNs

RT-SY2X-03+WOULRB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay ROX

- compatible for use on ABI Prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SY2X-03+WOUB

Table 4. qPCR Core kits and MasterMix Plus for SYBR® Green I use the hotstart enzyme, HotGoldStar. These kits can be adapted for use on 1 or 2-step reaction.

Product

Size

Catalog #

qPCR Core kit for SYBR® Assay No ROX

- compatible for use on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, iCycler iQ®, My iQ®, iQ5, DNA Engine Opticon® 1, DNA Engine Opticon® 2, Chromo 4, Mini Opticon®, Quantica®, LC480.

1000 RXNs

RT-SN10-05NR

qPCR Core kit for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

1000 RXNs

RT-SN10-05

qPCR Core kit for SYBR® Assay dTTP ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

1000 RXNs

RT-SN10-05NU

qPCR Core kit for SYBR® Assay Only dUTP ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

1000 RXNs

RT-SN10-05OU

qPCR MasterMix for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SN2X-03T

qPCR MasterMix Plus for SYBR® Assay No ROX

- compatible for use on Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480.

600 RXNs

RT-SN2X-03+NR

qPCR MasterMix Plus for SYBR® Assay No ROX + fluorescein

-fluorescein is needed to normalize the data correctly when using iCycler iQ®, iQ5 or My iQ® thermocyclers. Alternatively a separate tube of fluorescein is available in case you would like to use fluorescein in combination with any of our No ROX SYBR® Green I kits. This kit can be used on these machines.

600 RXNs

RT-SN2X-03+NRFL

qPCR MasterMix Plus for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

1200 RXNs

4000 RXNs

RT-SN2X-03+

RT-SN2X-06+

RT-SN2X-20+

qPCR MasterMix Plus dTTP for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXNs

RT-SN2X-03+WOUN

qPCR MasterMix Plus w/o UNG for SYBR® Assay Low ROX

- compatible for use on ABI 7500, Mx4000®, Mx3000P®, Mx3005P®.

600 RXNs

RT-SN2X-03+WOULR

qPCR MasterMix Plus w/o UNG for SYBR® Assay ROX

- compatible for use on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.

600 RXs

RT-SN2X-03WOU+

Click here to download the Manual for qPCR MasterMix Plus, qPCR Mastermix and qPCR Core Kit.

2012 Product Citations

MESA FAST for SYBR® Assays

Zong, Y. et al. PNAS 109, E3395 (2012).

Moreau, M. et al. Plant Cell 24, 463 (2012).

MESA Green for SYBR® Assays

Soto-Rifo, R. et al. Nucleic Acids Res 40, 2653 (2012).

Cobo, ER. et al. Infect Immun 80, 143 (2012).

Steinsiek, S. K. Bettenbrock, J Bacteriol 194, 5897 (2012).

MESA BLUE for SYBR® Assays

Rast, MI. and R. Simon, Plant Cell 24, 2917 (2012).

Bosch, D. and A. Saiardi, J Biol Chem 287, 38347 (2012).

Fichelson, P. et al. PNAS 109, 7893 (2012).

Core Kits

Zhang, L. et al. Plant Physiol 160, 1453 (2012).