MMP Antibodies, Assays & Enzymes


MMP Antibodies, Assays & Enzymes

AnaSpec, EGT Group's extensive range of MMP products includes the SensoLyte® MMP protease assay kits (FRET-based or colorimetric), antibodies, recombinant proteins and peptides. In response to popular demand to expand our SensoLyte® Plus line of kits, we are pleased to announce the recent release of the SensoLyte® Plus 520 MMP-2 assay kit. SensoLyte® Plus assays are designed specifically for detecting a certain MMP activity in biological sample which may contain multiple MMPs. A monoclonal anti-human-MMP antibody is employed to pull down the MMP of interest from the mixture first. The activity of the MMP is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-induced cleavage of the FRET substrate. SensoLyte® 520 MMP Assay Kits which do not contain a pull down antibody are useful for screening of inhibitors using a purified MMP. With published results in world-class journals and the industry's longest wavelength, the SensoLyte® Plus and SensoLyte® 520 line is one of the most sensitive (Figure 2) and brightest lines of MMP assay kits in the world.

SensoLyte® Plus 520 MMP-2 Assay

SensoLyteMMP2PlusSLAS2014poster

  • Measures specifically MMP-2 activity
  • Enhanced specificity – human capture antibody included
  • 96-well format (strips)
  • Mix and read
  • Fluorimetric - long wavelength
  • 5-FAM/QXL® 520 FRET pair (Ex/Em=490/520 nm)
  • To view SensoLyte® Plus 520 MMP-1, 9 & 13 assay kit information, please click here.
    Figure 1. SensoLyte® Plus 520 MMP-2 assay kit poster "A Fluorimetric Immunocapture Assay for Specific Detection of Active Matrix Metalloproteinase-2 in Biological Samples Using a 5-FAM/QXL® 520 FRET Peptide" presented at 2014 SLAS.
    SensoLyte® 520 MMP Assays
  • For screening of inhibitors using purified MMP
  • Measure MMP activity
  • 96-well or 384-well format
  • Mix and read
  • Fluorimetric - long wavelength
  • 5-FAM/QXL® 520 FRET pair (Ex/Em=490/520 nm)
  • Minimal autofluorescence
  • Assay time – as short as 1h
  • Figure 2. The performance of an identical peptide sequence, one coupled with 5-FAM/QXL® 520 and the other with EDANS/DABCYL FRET pairs was compared (A). A 5-FAM/QXL® 520 MMP substrate was compared with Mca/Dpa FRET substrate (B). Fluorescence was measured after 1 hr incubation of substrates using serial dilutions of MMP-13 (Flexstation 384II, Molecular Devices).


    Table 1. A listing of the MMP assay kits and recombinant MMPs.

    SensoLyte®520

    SensoLyte® Plus 520

    SensoLyte® 490 SensoLyte® ELISA

    Enzyme

    MMP-1

    71150

    72012

    71128

    72102

    55575*

    MMP-2

    71151

    72224

    71129

    72005

    MMP-3

    71152

    71130

    72103

    72006

    MMP-7

    71153

    71132

    72007

    MMP-8

    71154

    71133

    72104

    72008

    MMP-9

    71155

    72017

    71134

    72105

    55576*

    55884* (mouse)
    72069 (mouse)

    MMP-10

    72024

    72106

    72067

    MMP-12

    71157

    71137

    55525*

    MMP-13

    71156

    72019

    71135

    72107

    72011

    MMP-14

    72025

    72068

    MMP-15

    72198

    MMP-16

    72200

    MMP-24

    72201

    * From catalytic domain. These recombinant MMPs do not require pre-activation.

    MMP Antibodies

    Antibodies against MMP targets, with species reactivity towards human, mouse and rat; as well as zebrafish (Z-Fish™) are available through AnaSpec.

    Table 2. A listing of Z-Fish™ polyclonal anti-MMP antibodies, some of which are available in a smaller size (100 μl ) format.

    Cat#

    Size

    Product Name

    Application

    55345

    250 μl

    Anti-MMP-9 (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55345s

    100 μl

    55686

    250 μl

    Anti-MMP-11a (Catalytic Domain) Z-Fish™ antibody

    ELISA

    55687

    250 μl

    Anti-MMP-11a (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55687s

    100 μl

    55688

    250 μl

    Anti-MMP-11a (IN) Z-Fish™ antibody

    ELISA

    55689

    250 μl

    Anti-MMP-11b (Catalytic Domain) Z-Fish™ antibody

    ELISA

    55113

    250 μl

    Anti-MMP-11b (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55113s

    100 μl

    55690

    250 μl

    Anti-MMP-11b (NT) Z-Fish™ antibody

    ELISA

    55114

    250 μl

    Anti-MMP-13a (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55114s

    100 μl

    55115

    250 μl

    Anti-MMP-14a (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55115s

    100 μl

    55116

    250 μl

    Anti-MMP-14b (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55116s

    100 μl

    55117

    250 μl

    Anti-MMP-16a (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55117s

    100 μl

    55358

    250 μl

    Anti-MMP-17 (Hinge) Z-Fish™ antibody

    ELISA, WB

    55358s

    100 μl

    55403

    250 μl

    Anti-MMP-18 (Hinge) Z-Fish™ antibody

    ELISA

    55402

    250 μl

    Anti-MMP-18 (IN) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55402s

    100 μl

    55404

    250 μl

    Anti-MMP-21 (IN-2) Z-Fish™ antibody

    ELISA, WB

    55346

    250 μl

    Anti-MMP-24 (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55346s

    100 μl

    55405

    250 μl

    Anti-MMP-28 (Hinge) Z-Fish™ antibody

    ELISA, WB, IHC (whole mount)

    55405s

    100 μl

    Fig. 3. Zebrafish embryo (72 hpf) stained with Z-Fish™ anti-MMP-9 (Hinge), antibody (cat# 55345). Fig. 4. WB of Z-Fish™ anti-MMP-13a antibody (cat# 55114). An immunoreactive band at ~54 kDa was detected in zebrafish 24 hpf embryo lysate (A) and zebrafish 5 dpf lysate (B). Two bands appeared at ~80 kDa and ~45 kDa in zebrafish lysate (5 dpf) (B) indicate the proenzyme and the cleavage from MMP-13. All the bands were blocked by the immunizing peptide (C and D).


    Table 3. A listing of anti-MMP antibodies, human, mouse or rat specific.

    Cat#

    Product Name

    Type

    Species Reactivity

    Application

    54403

    Anti-MMP-1 (Hinge) antibody

    Rabbit polyclonal

    Human

    WB

    29574

    Anti-MMP-1 (NT) antibody

    Rabbit polyclonal

    Human

    ICC

    53674

    Anti-MMP -2 (72 kDa Collagenase IV) antibody

    Mouse monoclonal

    Human, mouse, rat

    WB, IHC-P

    29575

    Anti-MMP-2 (CT) antibody

    Rabbit polyclonal

    Human

    WB, IP

    55360

    Anti-MMP-2 (NT) antibody

    Rabbit polyclonal

    Mouse

    WB

    29577

    Anti-MMP-7 (CT) antibody

    Rabbit polyclonal

    Human, mouse

    WB, IHC

    29578

    Anti-MMP-8 (Hinge) antibody

    Rabbit polyclonal

    Human

    WB, ICC

    53678

    Anti-MMP-9 antibody

    Rabbit polyclonal

    Human, rat

    WB, IP

    54407

    Anti-human MMP-9 (Hinge)

    Rabbit polyclonal

    Human

    WB

    29579

    Anti-MMP-9 (Hinge-2)

    Rabbit polyclonal

    Human

    WB, ICC

    29580

    Anti-MMP-10 (Hinge)

    Rabbit polyclonal

    Human

    ICC, dot blot

    53670

    Anti-MMP-13 (Collagenase-3)

    Mouse monoclonal

    Human, mouse

    IHC-P, IP

    54406

    Anti-MMP-13 (Hinge)

    Rabbit polyclonal

    Human

    WB, IHC

    29581

    Anti-MMP-16 (IN)

    Rabbit polyclonal

    Human

    WB

    53675

    Anti-MMP-23

    Rabbit polyclonal

    Human

    IHC-P

    53379

    Anti-MMP-28 (Epilisin) (IN)

    Rabbit polyclonal

    Human

    WB

    Fig. 5. Human prostate carcinoma stained with rabbit polyclonal anti-MMP-7 (CT) antibody (Cat# 29577).

    Fig. 6. Human colon carcinoma (Formalin fixed / Paraffin-embedded) stained with rabbit anti-MMP-10 (Hinge) antibody, Cat# 29580, polyclonal antibody.
    Fig. 7. Human skin melanoma (Formalin fixed / Paraffin-embedded) stained with rabbit anti-MMP-13 (Hinge) antibody, Cat# 54406, polyclonal antibody.