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Histone H4 Peptides


AnaSpec, EGT Group is pleased to announce the expansion of our Histone H4 GO® (catalog) peptide collection. This, in addition to the wide selection of GO® Histone H3 peptides as well as Histone H1, H2A, H2B peptides, makes AnaSpec one of the world's largest providers of Histone peptides for the Epigenetics research community.


PTM histone sequences include:

  • Lysines
    • Acetylated
    • Mono-, di- , tri-methylated
    • Crotonylated
    • Ubiquitinated
  • Arginines
    • Mono-methylated, a/symmetrically di-methylated
    • Citrullinated
  • Serines or Threonines
    • Phosphorylated
  • Sequences with or without a biotin tag

Chromatin exist either as the transcriptionally active euchromatin or as the transcriptionally repressed heterchromatin state.1 Post-translational modifications of histone "tails" (amino termini) have been implicated in the conversion between the two states.1-2 Covalent modifications, such as acetylation, methylation and phosphorylation affect chromatin structure and exquisitely regulate gene expression.3-6 Histone hyperacetylation is correlated with increased transcription, whereas hypoacetylation correlates with transcriptional repression.5 In Histone H3, preferential methylation sites have been shown to be Lys4, Lys9, Lys27 and Lys36, with methylation generally leading to transcriptional suppression.3,7 Methylation of Histone H4 at Lys20 has also been found to repress transcription.8 Methylation of specific arginine residues in H3 has been shown to correlate with cell fate and potency.9 Phosphorylation at Ser10 (pSer10) is implicated in both transcription and cell division.10

Table 1. A listing of recently released H4 peptides.



Histone H4 Peptides

Size

Catalog #

[Arg(Me2s)3] - Histone H4 (1 - 21) - GGK(Biotin); H4R3(Me2s) (1 - 21), biotin – labeled
Ac - SG - R(me2s) - GKGGKGLGKGGAKRHRKV - GGK(biotin)

0.25 mg
1 mg
5 mg

AS-65424-025
AS-65424-1
AS-65424-5

[Lys(Ac)5/8/12/16] - Histone H4 (1 - 25) - GSGSK
SGRG - K(Ac) - GG - K(Ac) - GLG - K(Ac) - GGA - K(Ac) - RHRKVLRDNGSGSK

0.25 mg
1 mg

AS-65421-025
AS-65421-1

Histone H4 (8 - 30) - WGK(Biotin)
Ac - KGLGKGGAKRHRKVLRDNIQGIT - WGK(biotin)

0.25 mg
1 mg

AS-65417-025
AS-65417-1

[Lys(Me1)20] - Histone H4 (8 - 30) - WGK(Biotin); H4K20(Me1) (8 - 30), biotin - labeled
Ac - KGLGKGGAKRHR - K(Me1) - VLRDNIQGIT - WGK(biotin)

0.25 mg
1 mg

AS-65418-025
AS-65418-1

[Lys(Me2)20] - Histone H4 (8 - 30) - WGK(Biotin); H4K20(Me2) (8 - 30), biotin - labeled
Ac - KGLGKGGAKRHR - K(Me2) - VLRDNIQGITWG - K(biotin)

0.25 mg
1 mg

AS-65419-025
AS-65419-1

[Lys(Me3)20] - Histone H4 (8 - 30) - WGK(Biotin); H4K20(Me3) (8 - 30), biotin - labeled
Ac - KGLGKGGAKRHR - K(Me3) - VLRDNIQGITWG - K(biotin)

0.25 mg
1 mg

AS-65420-025
AS-65420-1

Table 2. A listing of recently released H3 peptides. Click headings to view the full listing of old and new H3 peptides.



Histone H3 Peptides

Size

Catalog #

Histone H3 (1-21)

[Lys (Me3) 4, Lys (Ac)9, pSer10] - Histone H3 (1 - 21)
ART - K(Me3) - QTAR - K(Ac) - pS - TGGKAPRKQLA

0.25 mg
1 mg

AS-65422-025
AS-65422-1

[Lys (Me3)4, Lys (Ac)9, pSer10] - Histone H3 (1 - 21) - GGK(Biotin)
ART - K(Me3) - QTAR - K(Ac) - pS - TGGKAPRKQLA - GGK(Biotin)

0.25 mg
1 mg

AS-65423-025
AS-65423-1

[Arg(Me2a)17, Lys(Ac)18] - Histone H3 (1 - 21) - GGK(Biotin); H3R17(Me2a)K14(Ac), biotin - labeled
ARTKQTARKSTGGKAP - R(me2a) - K(ac) - QLA - GGK(biotin) - NH2

0.25 mg
1 mg

AS-65414-025
AS-65414-1

Histone H3 (15-36)

Histone H3 (15 - 36) - GGK(Biotin)
APRKQLATKAARKSAPATGGVK - GGK(biotin)

0.25 mg
1 mg

AS-65415-025
AS-65415-1

[Arg(Me2s)26] - Histone H3 (15 - 36) - GGK(Biotin), H3R26(Me2s), biotin labeled
APRKQLATKAA - R(Me2s) - KSAPATGGVK - GGK(biotin)

0.25 mg
1 mg

AS-65416-025
AS-65416-1

Histone H3 (21-44)

[Lys(Ac)27, Lys(Me1)36] - Histone H3 (21 - 44) - GK(Biotin), H3K27(Ac)K36(Me1), biotin - labeled
ATKAAR - K(Ac) - SAPATGGV - K(Me1) - KPHRYRPG - GK(Biotin)

0.25 mg
1 mg

AS-65444-025
AS-65444-1

[Lys(Ac)27, Lys(Me2)36] - Histone H3 (21 - 44) - GK(Biotin), H3K27(Ac)K36(Me2), biotin - labeled
ATKAAR - K(Ac) - SAPATGGV - K(Me2) - KPHRYRPG - GK(Biotin)

0.25 mg
1 mg

AS-65445-025
AS-65445-1

[Lys(Me1)27/36] - Histone H3 (21 - 44) - GK(Biotin), H3K27(Me1)K36(Me1)
ATKAAR - K(Me1) - SAPATGGV - K(Me1) - KPHRYRPG - GK(Biotin)

0.25 mg
1 mg

AS-65446-025
AS-65446-1

Histone H3 (69-89)

Histone H3 (69 - 89), H3K79, amide
RLVREIAQDFKTDLRFQSSAV - NH2

1 mg

AS-65425

[Lys(Ac)79] - Histone H3 (69 - 89), H3K79(Ac), biotin - labeled
RLVREIAQDF - K(Ac) - TDLRFQSSAV - K(biotin)

0.25 mg
1 mg

AS-65441-025
AS-65441-1

[Lys(Me1)79] - Histone H3 (69 - 89), H3K79(Me1), amide
RLVREIAQDF - K(Me1) - TDLRFQSSAV - NH2

0.25 mg
1 mg

AS-65426-025
AS-65426-1

[Lys(Me1)79] - Histone H3 (69 - 89) - K(Biotin), H3K79(Me1), biotin labeled
RLVREIAQDF - K(Me1) - TDLRFQSSAV - K(Biotin)

0.25 mg

AS-64543

[Lys(Me2)79] - Histone H3 (69 - 89), H3K79(Me2), amide
RLVREIAQDF - K(Me2) - TDLRFQSSAV - NH2

0.25 mg
1 mg

AS-65427-025
AS-65427-1

[Lys(Me2)79] - Histone H3 (69 - 89) - K(Biotin), H3K79(Me2), biotin - labeled
RLVREIAQDF - K(Me2) - TDLRFQSSAV - K(Biotin)

0.25 mg

AS-64544

[Lys(Me3)79] - Histone H3 (69 - 89), H3K79(Me3), amide
RLVREIAQDF - K(Me3) - TDLRFQSSAV - NH2

0.25 mg
1 mg

AS-65428-025
AS-65428-1

[Lys(Me3)79]-Histone H3 (69-89)-K(Biotin), H3K79(Me3), biotin labeled
RLVREIAQDF - K(Me3) - TDLRFQSSAV-K(Biotin)

0.25 mg

AS-64545-025

Histone H3 (73-83)

Histone H3 (73 - 83)
EIAQDFKTDLR

1 mg
5 mg

AS-65437-1
AS-65437-5

[Lys(Ac)79] - Histone H3 (73 - 83), H3K79(Ac)
EIAQDF - K(Ac) - TDLR

1 mg
5 mg

AS-65438-1
AS-65438-5

[Lys(Me3)79] - Histone H3(73 - 83), H3K79(Me3)
EIAQDF - K(Me3) - TDLR

1 mg
5 mg

AS-65439-1
AS-65439-5

[Lys(biotin)79] - Histone H3 (73 - 83), H3K79(biotin), Biotinylated
EIAQDF - K(biotin) - TDLR

0.25 mg
1 mg

AS-65440-025
AS-65440-1

Table 3. A listing of recently released H2B peptides.



Histone H2B Peptides

Size

Catalog #

[Lys(cr)5] - Histone H2B(1 - 11), H2BK5(cr), crotonylated
PEPA - K(cr) - SAPAPK

0.25 mg
1 mg

AS-65443-025
AS-65443-1

[Lys(Ubi)120] - K(biotin) - W - Histone H2B (108 - 125); H2BK120(Ubi), C - Terminal biotin - labeled
K(biotin) - WKHAVSEGTKAVT - K(LRGG) - YTSSK

0.25 mg
1 mg

AS-65413-025
AS-65413-1

Please click below to view the full listing of Histone peptides. Contact biology@anaspec.com to obtain help to look up a certain peptide.
Histone H1 Peptides
Histone H2A Peptides
Histone H2B Peptides
Histone H3 Peptides
Histone H4 Peptides
Histone Related Substrates

Related Products

SensoLyte® Histone H3 (1 - 21) Sampler Kit *Biotinylated*
SensoLyte® Methylated Histone H3 (1 - 21) Sampler Kit *Biotinylated*
SensoLyte® 520 HDAC Assay Kit
SensoLyte® 440 HDAC Assay Kit
SensoLyte® 520 Deubiquitination Assay Kit
SensoLyte® 440 Deubiquitination Assay Kit
SensoLyte® 520 FRET SIRT1 Assay Kit
SensoLyte® Green SIRT1 Assay Kit
SensoLyte® 520 FRET SIRT2 Assay Kit
SensoLyte® Green SIRT2 Assay Kit
SensoLyte® HAT (p300) Assay Kit
SensoLyte® HAT (pCAF) Assay Kit
Monoclonal Antibody against 5-Methylcytidine
PTM Lysines & Arginines for Peptide Synthesis

References:
1. Grunstein, M. et al. J. Cell Sci. Suppl. 19, 29 (1995).
2. Strahl, BR and CD Allis, Nature 403, 41 (2000).
3. Strahl, BD. et al. Proc. Natl. Acad. Sci. 96, 14967 (1999).
4. Jenuwein, T. and CD. Allis Science 293, 1074 (2001).
5. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).
6. Lachner, M. et al. J. Cell Sci. 116, 2117 (2003).
7. Nishioka, K. et al. Genes & Dev. 16, 479 (2002).
8. Nishioka, K. et al. Mol. Cell 9, 1201 (2002).
9. Torres-Padilla, M-E. et al. Nature 445, 214 (2007).
10. Prigent, C. and S. Dimitrov, J. Cell Sci. 116, 3677 (2003).