Anti-β-Amyloid (1-6), (17-23) & (23-29) Antibodies - NEW
Anti-b-Amyloid (1-6), (17-23) & (23-29) Polyclonal Antibodies

AnaSpec is committed to supporting Alzheimer Researchers and this means continually offering new products that will aid in their quest to advance Alzheimer Disease Research. We are pleased to announce the release of three new polyclonal anti-human β-amyloid antibodies - Anti-β-Amyloid (1-6), (17-23) & (23-29) Polyclonal Antibodies.

Alzheimer's Disease (AD) is the most common neurodenerative disorder in elderly people. It has been demonstrated that AD has biological cause and is characterized by the presence of senile plaques and neurofibrillary tangles mainly in cerebral cortex and hippocampus brain regions.1-5 Amyloid-beta 1-40 (Aβ40) and amyloid-beta 1-42 (Aβ42) are the main components of the above plaques; however, other forms of amyloid-beta peptides also present. Both peptides are cleaved from the Amyloid Precursor Protein (APP) by β-secretase and γ-secretase enzymes.2,3,5 Many studies suggest that Aβ42 or/and Aβ43 are required to initiate formation of amyloid plaques and neurofibrills that leads to the neurodegeneration.1-5



Anti-β-Amyloid (1-6), (17-23) & (23-29) Polyclonal Antibodies - NEW

Anti-β-Amyloid (1-6), (17-23) & (23-29) antibodies are three new polyclonal β-amyloid antibodies recently released by AnaSpec. These rabbit polyclonal antibodies were raised against a synthetic peptides corresponding to 1-6, 17-23 and 23-29 amino acids of human β-amyloid, respectively.

Product

Size

Catalog #

Anti-Human β-Amyloid (1-6) Antibody, Rabbit Polyclonal

50 µg

AS-56074

Anti-Human β-Amyloid (17-23) Antibody, Rabbit Polyclonal

50 µg

AS-56075

Anti-Human β-Amyloid (23-29) Antibody, Rabbit Polyclonal - Amyloid monomer specific

50 µg AS-56076

Anti-Human β-Amyloid (1-6) Antibody, Rabbit Polyclonal Anti-Human β-Amyloid (17-23) Antibody, Rabbit Polyclonal Anti-Human β-Amyloid (23-29) Antibody, Rabbit Polyclonal
Figure 1. Human β-amyloid monomer (m), dimer (d) and fibrils (f) were probed with anti-β-amyloid (1-6) antibody (Cat# AS-56074) at 1 µg/ml final concentration. Secondary goat anti-Rabbit IgG-Hilyte™ Fluor 750 nm labeled antibody (Cat# AS-61056-05-H750 or AS-61056-1-H750) was used to visualize fluorescent band in the near-infrared part of the light spectra. Figure 2.Human β-amyloid monomer (m), dimer (d) and fibrils (f) were probed with anti-β-amyloid (17-23) antibody (Cat# AS-56075) at 1 µg/ml final concentration. Secondary goat anti-Rabbit IgG-Hilyte™ Fluor 750 nm labeled antibody (Cat# AS-61056-05-H750 or AS-61056-1-H750) was used to visualize fluorescent band in the near-infrared part of the light spectra. Figure 3. .Human β-amyloid monomer (m), dimer (d) and fibrils (f) were probed with anti-β-amyloid (23-29) antibody (Cat# AS-56076) at 1 µg/ml final concentration. Secondary goat anti-Rabbit IgG-Hilyte™ Fluor 750 nm labeled antibody (Cat# AS-61056-05-H750 or AS-61056-1-H750) was used to visualize fluorescent band in the near-infrared part of the light spectra.

Anti-β-Amyloid (1-40) & (1-42) Monoclonal Antibodies

Monoclonal anti-β-Amyloid (1-40) and (1-42) antibodies were raised from synthetic peptides corresponding to aa 35-40 (MVGGVV) and aa 35-42 (MVGGVVIA) of human β-Amyloid, respectively.

Product

Size

Catalog #

Anti-β-Amyloid (1 - 40), mouse monoclonal

50 µg

AS-55921

Anti-β-Amyloid (1 - 42), mouse monoclonal

50 µg

AS-55922

Anti-β-Amyloid (1-40), mouse monoclonal Figure 4. IHC of brain tissue from APP Tg2576 transgenic mouse probed with mouse anti-β-amyloid (1-40) antibody (Cat# AS-55921). Paraformaldehyde fixed sections were pretreated with 70% formic acid for 5 minutes followed by steaming for 30 minutes and blocked with normal goat serum (no need for antigen retrieval if fresh or frozen sections are used). Sections were incubated with anti-β-amyloid (1-40) antibody (Cat# AS-55921) at 0.5 µg/ml overnight. Immunoreactivity was visualized using DAKO Envision HRP System (1:10,000 dilution). This antibody stains cored plaques only (image courtesy of Mayo Clinic Florida).
Anti-β-Amyloid (1-42), mouse monoclonal Figure 5. IHC of brain tissue from APP Tg2576 transgenic mouse probed with mouse anti-β-amyloid (1-42) antibody (Cat# AS-55922). Paraformaldehyde fixed sections were pretreated with 70% formic acid for 5 minutes followed by steaming for 30 minutes and blocked with normal goat serum (no need for antigen retrieval if fresh or frozen sections are used). Sections were incubated with anti-β–amyloid (1-42) antibody (Cat# AS-55922) at 0.05 µg/ml concentration overnight. Immunoreactivity was visualized using DAKO Envision HRP System (1:10,000 dilution). This antibody stains both cored and diffused plaques (image courtesy of Mayo Clinic Florida).

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References:

1. Levites, Y. et al. J Clin Invest 116, 193 (2006).
2. Broersen, K. et al. Alzheimer's Res Ther 2, 1 (2010).
3. Zhang, Y-W. et al. Mol Brain 4, 1 (2011).
4. Koechling, T. et al. Int J Alzheimer's Dis (2010).
5. Bobba, A., et al. Int J Alzheimer's Dis (2010).