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Research Topics
HiLyte Fluor™ Far Red to NIR Dyes - Revolutionary


Knowingly or unknowingly, researchers have become accustomed to paying exorbitant costs for high-performance dye products in the far red and near infrared spectrum. Utilizing its proprietary HiLyte Fluor technology, AnaSpec introduces a collection of detection solutions that are higher performing AND more cost effective. Let the revolution begin.

AnaSpec’s HiLyte Fluor™ fluorescent dyes in the far red and near infrared offer more sustained fluorescence over time than other leading brands. Combining better performance with better value, HiLyte Fluor dyes are truly revolutionary.



Figure 1: Comparison of fluorescence with exposure to white light.

Applications

AnaSpec’s HiLyte Fluor dyes are ideal for labeling secondary antibodies and streptavidin in the far red and near infrared spectrum. Preliminary data has also indicated the effectiveness of HiLyte Fluor 750 labeled peptides in live, small animal imaging studies.

Far Red to Near Infrared Secondary Antibodies

ProductGoat anti-mouse IgG (H+L)Goat anti-rabbitIgG (H+L)Rabbit anti-goatIgG (H+L) Rabbit anti-chickenIgY (H+L)
HiLyte Fluor™ 647-labeled 28175-H647 28176-H647 28168-H647 29709-H647
HiLyte Fluor™ 680 labeled 28175-H680 28176-H680 28168-H680 29709-H680
HiLyte Fluor™ 750 labeled 28175-H750 28176-H750 28168-H750 29709-H750












More Fluorescent Secondary Antibodies

Tubulin in human glioblastoma cells was detected with mouse-anti-tubulin primary antibody, visualized with HiLyte Fluor™ 647-conjugated rabbit-anti-mouse IgG (Cat#28164-H647). Nuclei were stained with Hoechst 33342 (Cat# 83218).

Far Red to Near Infrared Streptavidin-Dye Conjugates

Product NameCat#Abs/Em (nm)
Streptavidin, HiLyte FluorTM 647 conjugated 60667 650/668
Streptavidin, HiLyte FluorTM 680 conjugated 60659-H680 678/699
Streptavidin, HiLyte FluorTM 750 conjugated 60659-H750 754/778








Other Labeled Streptavidin Products

Product NameCat#Abs/Em (nm)
Streptavidin, APC conjugated 60661 652/662
Streptavidin, RPE conjugated cited in a 2006 paper1 60669 565/575
Streptavidin, 5-FAM conjugated 60664 565/575
Streptavidin, HiLyte FluorTM 488 conjugated 60665 495/524
Streptavidin, HiLyte FluorTM 555 conjugated 60666 555/565











References:
1. Vergote, D. et al. Proc. Natl. Acad. Sci. 103, 19182 (2006).