New Cytokine Antibodies - Interleukin & Chemokine


With an aim towards providing our customers with more antibody choices specifically targeted for their area of interest, AnaSpec is pleased to announce the addition of hundreds of antibodies to our existing portfolio. Recognizing that most labs only need a small amount of antibody at a time, AnaSpec is pleased to introduce a new, more economical 50 µg size format in order to optimize our customers’ cost efficiency. This month we focus on our newest cytokine antibodies – interleukin & chemokine.

Interleukin

Interleukin-32 (IL-32), whose expression is increased following activation by IL-2, was initially identified as a transcript (NK4) selectively expressed in lymphocytes and NK cells.1 It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32.2 IL-32 does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-a, IL-8 and MIP-2, and because it is induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN-g, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.3 Rabbit anti-IL-32 polyclonal antibody was raised against a 15 amino acid peptide from near the C-terminus of human IL-32 (Genbank accession No. AAH09401). Anti-IL-32 reacts with IL-32 at the molecular weight of approximately 22-25 kDa on western blot. Anti-IL-32 detects all isoforms of IL-32.

For a full listing of all interleukin antibodies, please click here

Chemokine

G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 have been identified as HIV coreceptors.4 These coreceptors, as well as CD4, are needed to infect target cells. CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells.5,6 It is required for infection by the dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2.7,8 The a -chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1.9,10 CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells.11 CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types.2 Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells.5,8,13 The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.13,14 Rabbit polyclonal CXCR4 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human CXCR4.6,12

For a full listing of chemokine antibodies, click here

To request AnaSpec’s Detection Reagents & Kits catalog, click here

REFERENCES:
1. Dahl, CA. et al. J. Immunol. 148, 597 (1992).
2. Kim, SH. et al. Immunity 22, 131 (2005).
3. Cagnard, N. et al. Eur. Cyto. Network 16, 289 (2005).
4. Dimitrov DS. Cell 91, 721 (1997).
5. Feng, Y. et al. Science 272, 872 (1996).
6. Berson, JF. et al. J. Virol. 70, 6288 (1996).
7. Doranz, BJ. et al. Cell 85, 1149 (1996).
8. Endres, MJ. et al. Cell 87, 745 (1996).
9. Bleul, CC. et al. Nature 382, 829 (1996).
10. Oberlin, E. et al. Nature 382, 833 (1996).
11. Lapham, CK. et al. Science 274, 602 (1996).
12. Leoetscher, M. et al. J. Biol. Chem. 269, 232 (1994).
13. Brelot, A. et al. J. Virol. 71, 4744 (1997).
14. Lu, Z. et al. Proc. Natl. Acad. Sci. USA 94, 6426 (1997).