NEW Labeled Antibodies: Anti-CD-3ε, Anti-CD79α


Leveraging its expertise in both detection reagents and antibodies, AnaSpec is pleased to introduce a new series of labeled anti-CD-3ε and anti-CD79a antibodies. With a range of classic labels like biotin, FAM, and FITC as well as AnaSpec’s proprietary HiLyte™ Fluor labels, AnaSpec’s line of anti-CD-3ε , anti-CD79α antibodies provide a research-ready spectrum of detection possibilities for use in applications such as flow cytometry, immunohistochemistry and fluorescent in-situ hybridization.

Custom labeling service for all our primary antibodies is available. Available labels include HiLyte™ Fluor™ dyes, HRP, AP, biotin, R-PE (R-phycoerythrin) and B-PE (B-phycoerythrin). Please inquire at service@anaspec.com

Click here to request AnaSpec’s Detection Reagents & Kits catalog.

Anti-CD-3ε

T cell activation through the T cell antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3g, CD3d, CD3e and CD3z.1,2 These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11.1 The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3e may play a role in TCR-induced growth arrest, cell survival and proliferation.3 Rabbit anti-CD3e polyclonal antibody was raised against a synthetic peptide from the human CD3 protein, epsilon chain, only one amino acid different from mouse and rat origins.

Cat#

Form

Unit Size

29588

Unconjugated

50 mg

29588-BIOT

Biotinylated

50 mg

29588-FAM

FAM-labeled

50 mg

29588-TAMR

TAMRA-labeled

50 mg

29588-AMCA

AMCA-labeled

50 mg

29588-FITC

FITC-labeled

50 mg

29588-H488

HiLyte™ Fluor 488-labeled

50 mg

29588-H555

HiLyte™ Fluor 555-labeled

50 mg

29588-H647

HiLyte™ Fluor 647-labeled

50 mg

29588-H750

HiLyte™ Fluor 750-labeled

50 mg








IHC of Human tonsil probed with anti-CD3ε (Cat# 29588).

Anti-CD-79α

The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79α) and B29 (CD79β), is physically associated in the B-cell membrane with immunoglobulins to constitute the B cell antigen receptor (BCR).4,5 The CD79α/β heterodimer interacts with at least one tyrosine kinase (Lyn).6 Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79α/β dimer, and also of other molecules, thereby initiating intracellular signaling.6 Almost all acute lymphoblastic leukemias of precursor B cell type are positive when tested with CD79α antibodies.7-9 Rabbit anti-CD79α polyclonal antibody was raised against a synthetic peptide corresponding to human and mouse CD79α.






IHC of Human tonsil probed with anti-CD79α(Cat# 29560).


Cat#

Form

Unit Size

29560

Unconjugated

50 mg

29560-BIOT

Biotinylated

50 mg

29560-FAM

FAM-labeled

50 mg

29560-TAMR

TAMRA-labeled

50 mg

29560-AMCA

AMCA-labeled

50 mg

29560-FITC

FITC-labeled

50 mg

29560-H488

HiLyte™ Fluor 488-labeled

50 mg

29560-H555

HiLyte™ Fluor 555-labeled

50 mg

29560-H647

HiLyte™ Fluor 647-labeled

50 mg

29560-H750

HiLyte™ Fluor 750-labeled

50 mg

Custom Labeling Service Available for the following:

Label

Abs/Em

AP

N/A

HRP

N/A

Biotin

N/A

AMCA

353/442

FAM

492/518

FITC

493/517

TAMRA

542/568

HiLyte™ 488

498/525

HiLyte™ 555

552/569

HiLyte™ 647

649/674

HiLyte™ 750

753/778

R-PE

565/575

B-PE

545/575

Related Products:

AnaTag Labeling Kits

To view a recent poster on HiLyte™ Fluor 750, please click here.

References:

1. Clevers, H. et al. Annu. Rev. Immunol. 6, 629(1988).
2. Malissen, B. et al. Curr. Opin. Immunol. 5, 324 (1993).
3. Gil, D. et al. J. Biol. Chem. 276, 11174 (2001).
4. Mason, DY. et al. Blood 86, 1453 (1995).
5. Payelle-Brogard, B. et al. Blood 94, 3516 (1999).
6. Chu, PG. et al. Appl. Immunohistochem. Mol. Morphol. 9, 97 (2001).
7. Verschuren, MCM. et al. Leukemia 7, 1931 (1993).
8. Buccheri, V. et al. Blood 82, 853 (1993).
9. Payelle-Brogard, et al. Br. J. Hematology 118, 976 (2002).