Recent articles by B. Turk et al.^1-2 reported the use of positional scanning synthetic peptide combinatorial libraries (PS-SPCL)³ for rapid identification of serine/threonine kinase phosphorylation motif. As the key supplier of kinase peptide libraries mentioned in the articles, AnaSpec is pleased to announce the worldwide commercial availability of its newest kinase peptide library.

Protein kinases play an important universal role in the regulation of signal transduction pathways by catalyzing the reversible phosphorylation of protein substrates. To date, 518 putative protein kinase genes have been identified, but not all have been characterized.⁴ Profiling the sequence preference of different kinases will help in understanding the roles played by the different kinases as well as identification of potential inhibitors.¹

The library consists of 198 distinct peptide mixtures. “In each mixture, one of the 20 naturally occurring proteogenic amino acids was fixed at each of 9 positions surrounding a central serine/threonine residue.” ²

Product	Size	Catalog #
Kinase Substrates Library, Group I, biotinylated, 180 peptides	1 mg x 180 vials	62017-1
Kinase Substrates Library, Group I, biotinylated, 180 peptides	5 mg x 180 vials	62017-5
Kinase Substrates Library, Group II, biotinylated, 18 peptides	1 mg x 18 vials	62335

The following shows how these peptides are configured:

Group I:

Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-Z-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-Z-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-Z-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-X-Z-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-X-X-S/T-Z-X-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-X-X-S/T-X-Z-X-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-X-X-S/T-X-X-Z-X-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
Y-A-X-X-X-X-X-S/T-X-X-X-Z-A-G-K-K(LC-Biotin)-NH₂, 20 peptides
X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine sidechain.
S/T = equimolar mixture of serine and threonine.
Z = one of the 20 naturally occurring amino acids fixed at this postiion

Group II:

Y-A-pT-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-pT-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-pT-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-pT-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-pT-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-pT-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-pT-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-pT-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-X-pT-A-G-K-K(LC-biotin)-NH₂
Y-A-pY-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-pY-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-pY-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-pY-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-pY-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-pY-X-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-pY-X-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-pY-X-A-G-K-K(LC-biotin)-NH₂
Y-A-X-X-X-X-X-S/T-X-X-X-pY-A-G-K-K(LC-biotin)-NH₂
X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine.
LC = "long chain" version with an additional aminohexanoic acid spacer between the biotin and lysine sidechain.
S/T = equimolar mixture of serine and threonine. pT is phosphothreonine, and pY is phosphotyrosine.

Contact service@anaspec.com for more information regarding the synthesis of these or any other peptide libraries.

References:

1. Turk, BB. et al. Nature Protocols 1, 375 (2006).
2. Hutti, JE. et al. Nature Methods 1, 27 (2004).
3. Pinilla, C. et al. Biotechniques 13, 901 (1992).
4. Manning, G. et al. Science 298, 1912 (2002).