Continuing to provide researchers
with an ever-expanding collection of research and drug discovery tools, AnaSpec
is pleased to introduce two new catalog antibodies: Anti-Keratin-18 and Anti-Keratin-19.
Intermediate filaments (IFs), together with actin microfilaments
and tubulin microtubules, comprise the three major cytoskeletal networks that
are found in most eukaryotic cells.1 Keratins, which are the largest
IF protein subgroup, can be divided into the acidic type I (K9-K20) and basic
type II (K1-K8) keratins.2 Typically, epithelial cells express
at least one of each type, and the proteins exist in the cells as noncovalent
obligate heteropolymers. Keratin networks are highly dynamic and reorganized
during cell differentiation, mitosis and apoptosis, and also play important
cytoprotective and structural support roles for the cell.3,4 During
apoptosis, the epithelial keratins 18/19 undergo caspase-mediated proteolysis,
leading to the disassembly of the nuclear envelope and cytoplasmic IF networks.5
K18 and K19 undergo caspase-cleavage at the conserved Asp237 in their rod
domains.5 In contrast to other type I keratins, K18 is unique in
that it includes another caspase-cleavage site at Asp396 of the carboxy-terminal
Rabbit anti-human K18 (IN-2) polyclonal antibody was raised
against a synthetic peptide corresponding to an internal sequence of human
keratin-18. This epitope affinity purified rabbit polyclonal antibody reacts
predominantly with human keratin-18. Western blot using HT29 cell lysate from
human colonic carcinoma shows a 49 kDa immunoreactive protein. Human, mouse,
and monkey reactive. Predicted to detect dog, bovine, and frog keratin -18.
Rabbit anti-human K19 (IN) polyclonal antibody was raised against
a synthetic peptide corresponding to an internal sequence of human keratin-19.
This epitope affinity purified rabbit polyclonal antibody reacts predominantly
with human keratin-19. Western blot using HT29 cell lysate from human colonic
carcinoma shows a ~45 kDa immunoreactive protein. Human, mouse, chimpanzee,
and monkey reactive. Predicted to detect rat and bovine keratin-19.
||Human K-18 probed
with anti-K18 at 1 ug/ml.
||Human K-19 probed with
anti-K19 at 1 ug/ml.
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Anti-Actin; smooth muscle
1. Ku, NO. et al. Am. J. Physiol.
277, G1108 (1999).
2. Schweizer, J. et al. J. Cell. Biol.
174, 169 (2006).
3. Omary, MB. et al. et al.
Trends Biochem. Sci. 31, 383 (2006).
4. Omary, MB. et al. N. Engl. J. Med. 351, 2087 (2004)
5. Ku, NO. et al. J. Biol. Chem.
272, 33197 (1997).
6. Ku, NO. and MB. Omary, J. Biol. Chem.
276, 26792 (2001).