In head to head comparisons between HiLyte Fluor™
dyes and comparable dyes such as Cy® & others, HiLyte Fluor™
dyes have delivered superior results that can best be described as brilliant.
With the new HiLytePlus™ series of long wavelength dyes, brilliant gets even
AnaSpec is pleased to introduce HiLytePlus™.
Built on HiLyte Fluor™ technology, the HiLytePlus™ series represents AnaSpec’s highest performing dyes
for Red-NIR wavelengths.
- Even brighter than HiLyte Fluor™ dyes
- Like HiLyte Fluor™ series, outperforms Cy® & DyLight™ dyes
- Excitation wavelengths of 555, 647 and 750
- Ideal for high-performance biomarker labeling
- Available as individual dyes, AnaTag™ Labeling
Kits, labeled secondary antibodies, and labeled streptavidin.
Goat anti-rabbit antibody was labeled with
a mole ratio of 8:1 of each of the indicated dyes in to give a degree of substitution
of 3 (+/- 8%). Solutions of rabbit IgG were added to the wells of high-binding
96-well plates, which were then blocked with BSA. A solution of the labeled
antibodies were added and the plates, after incubation and washing, were read
at the optimal excitation and emission wavelengths.
These dyes are available in different reactive forms – acid, amine, C2 maleimide,
hydrazide, and succinimidyl esters, SE. The SE reactive form is especially
useful for labeling amino groups on proteins and appropriately modified nucleic
acids using standard, familiar labeling protocols.
Performance without compromise. Value without sacrifice.
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