The labeling of proteins
and antibodies with fluorescent dyes is crucial for researchers who use flow
cytometry, immunohistochemistry and fluorescent in-situ hybridization techniques.
AnaSpec’s AnaTagTM
Labeling Kits are now available with the revolutionary new HiLytePlus™ series of long-wavelength dyes.
Built on HiLyte Fluor™ technology, the HiLytePlus™ series represents AnaSpec’s highest
performance dyes for Red-NIR wavelengths. A perfect marriage of superior convenience and brilliant
performance, AnaTag™ HiLytePlusTM Protein Labeling Kits are
ideal for long wavelength, cost-effective, biopolymer labeling.
Superior Performance
•
Available for the popular excitation wavelengths of 555, 647 and 750 nm
•
Protein conjugates brighter than Cy3, and Cy5 protein conjugates
• Performance levels even higher than HiLyte FluorTM dyes
Figure 1.The succinimidyl ester (SE)
reactive form of the labeling dye reacts with the amine group on the protein
and forms stable carboxamide bonds which are identical to and as stable as
the natural peptide bond. These dye-protein conjugates are very stable and
are suitable for immunofluorescent staining, fluorescence in situ hybridization,
flow cytometry and other biological applications.
Ultra-Convenient
•
Fast Labeling time - as short as 30 minutes
•
All essential components for conjugation reaction and purification of dye-protein
conjugates
•
Available in two convenient reaction sizes:
Microscale labeling kit: up to 200 ug protein x
3 labelings or
Larger scale labeling kit: up to 5 mg protein x
3 reactions
•
Stable dye-protein conjugate products
• Industry-standard amine-reactive chemistry
|
|
HiLytePlus™ 555 |
HiLytePlus™ 647 |
HiLytePlus™ 750 |
| Large
scale
(up to 5 mg x 3 labelings) |
72091 |
72093 |
72089 |
Microscale
(up to 200 ug x 3 labelings) |
|
|
|
