In response to customer inquiries, AnaSpec has expanded its catalog of MMP
assay kits to include a new colorimetric assay kit. The pioneer of sensitive,
long wavelength fluorometric MMP assay kits, AnaSpec is pleased to introduce
our first quantitative and homogeneous colorimetric MMP assay kit.
Generic MMP Assay Kit can be used for the continuous spectrophotometric assay
of MMP activity (including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14) and for the
high throughput screening of MMP inducers and inhibitors.
substrate is a thiopeptide that is cleaved by the MMPs, releasing a sulfhydryl
group. The sulfhydryl group reacts with Ellman’s Reagent (5,5’-dithiobis(2-nitrobenzoic
acid). The final product of this reaction, 2-nitro-5-thiobenzoic acid (TNB),
can be detected at 412 nm using a microplate reader. The assays are performed
in a convenient 96-well microplate format.
here to request additional literature
Figure 1. GM6001 inhibition of MMP-9 activity measured with SensoLyte™
Generic MMP Assay Kit.
Assay Kits and Recombinant MMPs
MMP FRET Peptide
Substrates and MMP Related Peptides
MMP Biotinylated Substrate
MMP Peptide Inhibitor
CTT, Gelatinase Inhibitor
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