In response to customer inquiries, AnaSpec has expanded its catalog of MMP
assay kits to include a new colorimetric assay kit. The pioneer of sensitive,
long wavelength fluorometric MMP assay kits, AnaSpec is pleased to introduce
our first quantitative and homogeneous colorimetric MMP assay kit.
The SensoLyte™
Generic MMP Assay Kit can be used for the continuous spectrophotometric assay
of MMP activity (including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14) and for the
high throughput screening of MMP inducers and inhibitors.
The chromogenic
substrate is a thiopeptide that is cleaved by the MMPs, releasing a sulfhydryl
group. The sulfhydryl group reacts with Ellman’s Reagent (5,5’-dithiobis(2-nitrobenzoic
acid). The final product of this reaction, 2-nitro-5-thiobenzoic acid (TNB),
can be detected at 412 nm using a microplate reader. The assays are performed
in a convenient 96-well microplate format.
Click
here to request additional literature
Figure 1. GM6001 inhibition of MMP-9 activity measured with SensoLyte™
Generic MMP Assay Kit.
Related products:
SensoLyte™ MMP
Assay Kits and Recombinant MMPs
MMP FRET Peptide
Substrates and MMP Related Peptides
MMP Biotinylated Substrate
I
MMP Peptide Inhibitor
I
CTT, Gelatinase Inhibitor
MMP
Antibodies
References:
1. Woessner, J.F. Jr. and CJ.
Taplin, J. Biol. Chem. 263, 16918 (1988).
2. Woessner, JF. Jr. FASEB
J. 5, 2145 (1991).
3. Gravallese, EM. et al.
Arthritis Rheum. 34, 1076 (1991).
4. Stetler-Stevenson, WG. et al.
Annu. Rev. Cell Biol. 9, 541 (1993).
5. Goldberg, GI. et al. Ann.
NY. Acad. Sci. 580, 375 (1990).
