NEW SensoLyte™ Colorimetric MMP Assay Kit


In response to customer inquiries, AnaSpec has expanded its catalog of MMP assay kits to include a new colorimetric assay kit.  The pioneer of sensitive, long wavelength fluorometric MMP assay kits, AnaSpec is pleased to introduce our first quantitative and homogeneous colorimetric MMP assay kit.

The SensoLyte™ Generic MMP Assay Kit can be used for the continuous spectrophotometric assay of MMP activity (including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14) and for the high throughput screening of MMP inducers and inhibitors.

The chromogenic substrate is a thiopeptide that is cleaved by the MMPs, releasing a sulfhydryl group. The sulfhydryl group reacts with Ellman’s Reagent (5,5’-dithiobis(2-nitrobenzoic acid). The final product of this reaction, 2-nitro-5-thiobenzoic acid (TNB), can be detected at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.

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Product

Size

Catalog #

SensoLyte™ Generic MMP Assay Kit “Colorimetric”  NEW

1 kit

72095



Figure 1.  GM6001 inhibition of MMP-9 activity measured with SensoLyte™ Generic MMP Assay Kit. 

Related products:

SensoLyte™ MMP Assay Kits and Recombinant MMPs
MMP FRET Peptide Substrates and MMP Related Peptides
MMP Biotinylated Substrate I
MMP Peptide Inhibitor I
CTT, Gelatinase Inhibitor
MMP Antibodies

References:

1.  Woessner, J.F. Jr. and CJ. Taplin, J. Biol. Chem. 263, 16918 (1988).
2.  Woessner, JF. Jr. FASEB J. 5, 2145 (1991).
3.  Gravallese, EM. et al. Arthritis Rheum. 34, 1076 (1991).
4.  Stetler-Stevenson, WG. et al. Annu. Rev. Cell Biol. 9, 541 (1993).
5.  Goldberg, GI. et al. Ann. NY. Acad. Sci. 580, 375 (1990).