The detection of proteases
and screening of specific protease inhibitors are critical factors in the
discovery of potential drugs for the treatment and management of protease-related
diseases. In the last decade or so, the use of fluorescence resonance energy
transfer (FRET) technology has made the continuous assay of protease activity
and high throughput screening (HTS) of protease inhibitors faster and easier.
AnaSpec’s SensoLyte™ line offers the industry’s first and most
sensitive collection of long wavelength protease assay kits. Combining proprietary
FRET technologies with world-class peptide expertise, the SensoLyte™ 520 family demonstrates significantly superior performance over traditional
assay kits. The perfect overlap of absorption and emission spectrums of the
SensoLyte™ FRET pairs (Figures 1
and 2) allows for a level of sensitivity that is as much as 40x greater.