The detection of proteases and screening of specific protease inhibitors are critical factors in the discovery of potential drugs for the treatment and management of protease-related diseases.  In the last decade or so, the use of fluorescence resonance energy transfer (FRET) technology has made the continuous assay of protease activity and high throughput screening (HTS) of protease inhibitors faster and easier.

AnaSpec’s SensoLyte™ line offers the industry’s first and most sensitive collection of long wavelength protease assay kits.  Combining proprietary FRET technologies with world-class peptide expertise, the SensoLyte™ 520 family demonstrates significantly superior performance over traditional assay kits.  The perfect overlap of absorption and emission spectrums of the SensoLyte™ FRET pairs  (Figures 1 and 2) allows for a level of sensitivity that is as much as 40x greater.

Figure 1.  The absorption spectrum of QXL™ 520 perfectly overlaps with the emission spectrum of 5-FAM.	Figure 2.  The absorption spectrum of QXL™ 520 perfectly overlaps with the emission spectrum of HiLyte Fluor™ 488.

Information regarding these kits can be found in the newly-released FRET Technology Brochure.  The brochure features:

·  Protease assay kits for HTS of inhibitors or quantitation of HCV, HIV, MMPs, a and b-Secretases, Renin, TACE, ACE2, & WNV. 

·  Table of the different donor and acceptor molecules

·  Listing of dye-labeled amino acids or resins that can be used as building blocks

·  Concise guideline on how to design FRET peptides

Click here to request AnaSpec’s FRET Technology Brochure

Related Products and Services:

FRET peptides
All assay kits
Price quotation for custom synthesis of FRET or non-FRET peptides
Dye-labeled amino acids & resins