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| Fluo-3 - High Affinity Fluorescent Calcium Indicator |
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Calcium plays a vital role in the specific and selective regulation
of major cellular processes and is central in the regulation
of cardiac contractility, growth and gene expression. Calcium
indicators are small molecules that can respond to the binding
of Ca2+ ions by changing their spectral properties. Recognizing
the need for versatile tools for the detection of calcium, AnaSpec is pleased
to highlight Fluo-3, a high affinity fluorescent calcium indicator.
Fluo-3 absorbs at approximately 506 nm and emits at 526 nm when
bound to calcium. In the absence of calcium it is practically non-fluorescent.
The higher Kd and longer excitation wavelength of Fluo-3 can have significant
advantages over other indicators because of the reduced interference with
sample autofluorescence. It demonstrates efficient excitation with laser-based
instrumentation, including confocal laser scanning microscopes. It has been
widely used for flow cytometry and is one of the imaging methods of intracellular
calcium flux that is associated with GPCR dynamics.
Request AnaSpec’s Detection Reagents & Kits catalog
today. Click here
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