SensoLyteTM MMP Assay Kits – The New Industry Standard


In less than 3 years since their introduction, AnaSpec’s SensoLyte™ line of MMP assay kits have quickly become the new standard in MMP assay sensitivity. With published results in world-class journals and the industry’s longest wavelength, the SensoLyte™ 520 line is one of the most sensitive (see Figure 1) and brightest lines of MMP assay kits in the world.

SensoLyte™ MMP assay kits can be used to detect the activity of MMPs in serum, plasma, synovial fluids, supernatants of cell culture media, tissue homogenates, and sputum. They are also ideal for the screening of MMP inducers and inhibitors.


SensoLyte™ 520 MMP kits incorporate a 5-FAM/QXL520™ labeled FRET peptide substrate. With superior fluorescence quantum yield and longer emission wavelength, these FRET peptides more effectively avoid the autofluorescence of test compounds.

Addressing the poor substrate sequence specificity of certain members of the MMP family, SensoLyte Plus™ MMP assay kits employ a novel immuno-capture technique for researchers who need to identify the activity of a specific MMP in a complex biological sample containing multiple MMPs.

Request AnaSpec’s new FRET Probes Brochure today



Figure 1. An MMP generic FRET substrate containing 5-FAM/QXL™ 520 shows higher sensitivity than the EDANS/DABCYL based FRET substrate.


Figure 2. The SensoLyte™ 520 Generic Assay Kit detects the activity of several MMPs. APMA-activated MMPs, 30 ng each, were incubated with 5-FAM/QXL™ 520 FRET substrate. Fluorescence was monitored 1h after the start of the reaction at Ex/Em=490 nm/520 nm.

 

SensoLyte™ 520 Series

SensoLyte Plus™ 520 Series  

Recombinant Human MMPs

 

Cat# / lowest detection concentration

Cat# / lowest detection concentration

Cat#

MMP-1

71150

72012

72004

0.5 ng

0.03 ng

MMP-2

71151

 

72005

1.5 ng

MMP-3

71152

 

72006

2 ng

MMP-7

71153

 

72007

1 ng

MMP-8

71154

 

72008

0.2 ng

MMP-9

71155

72017

72009

0.02ng

0.3 ng

72069 (mouse)

MMP-10

72024

 

72067

0.8 ng

MMP-12

71157

 

72010

1 ng

MMP-13

71156

72019

72011

3 ng

0.3 ng

MMP-14

72025

 

72068

0.2 ng

Related Products and Services:

Product

Cat #

SensoLyte™ 520 Generic MMP Assay Kit *Fluorimetric*

71158

SensoLyte™ 520 MMP Profiling Kit *Fluorimetric*

71136

SensoLyte™ 520 MMP Substrate Sampler Kit *Fluorimetric*

71170

SensoLyte™ Generic MMP Assay Kit *Colorimetric*  NEW

72095

SensoLyte™ 490 MMP Assay Kits *Fluorimetric* (Ex/Em=340 nm/490 nm)