In less than 3 years since their introduction, AnaSpec’s SensoLyte™
line of MMP assay kits have quickly become the new standard in MMP assay sensitivity.
With published results in world-class journals and the industry’s longest
wavelength, the SensoLyte™ 520 line is
one of the most sensitive (see Figure 1) and brightest lines of MMP assay
kits in the world.
SensoLyte™ MMP assay kits can be used to detect the activity of
MMPs in serum, plasma, synovial fluids, supernatants of cell culture media,
tissue homogenates, and sputum. They are also ideal for the screening of MMP
inducers and inhibitors.
SensoLyte™ 520 MMP kits incorporate a 5-FAM/QXL520™ labeled FRET
peptide substrate. With superior fluorescence quantum yield and longer emission
wavelength, these FRET peptides more effectively avoid the autofluorescence
of test compounds.
Addressing the poor substrate sequence specificity of certain members of
the MMP family, SensoLyte Plus™ MMP assay kits employ a
novel immuno-capture technique for researchers who need to identify the
activity of a specific MMP in a complex biological sample containing multiple
Request AnaSpec’s new FRET Probes Brochure
Figure 1. An MMP generic FRET substrate containing 5-FAM/QXL™
520 shows higher sensitivity than the EDANS/DABCYL based FRET substrate.
Figure 2. The SensoLyte™ 520 Generic Assay Kit detects the
activity of several MMPs. APMA-activated MMPs, 30 ng each, were incubated
with 5-FAM/QXL™ 520 FRET substrate. Fluorescence was monitored 1h after the
start of the reaction at Ex/Em=490 nm/520 nm.
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