New Applications for MMP Substrates


Leveraging AnaSpec’s proprietary detection technologies, AnaSpec FRET-based MMP substrates were recently used in a double differential in vivo zymography study at the University of New Brunswick. The successful results demonstrate the breadth of applications in which AnaSpec’s innovative products can be utilized.

A mixture of QXL™ 570/5-TAMRA-labeled Peptide I incorporating a red fluorophore, and QXL™ 520/5-FAM-labeled Peptide XIII incorporating a green fluorophore were used to detect differential breakdown of the two different MMP substrates in zebrafish. Peptide I was broken down mostly in the maturing myotome boundaries, whereas Peptide XIII was broken down much more strongly in the notochord sheath (Courtesy of Dr. Bryan Crawford, Univ. of New Brunswick, Fredericton, NB).

Synthetic FRET peptide substrates provide a reliable method for monitoring enzyme activity by following substrate cleavage. Through AnaSpec’s innovative FRET technologies, the SensoLyte™line of assay kits offers detection of MMPs and other substrates at the industry’s longest wavelengths. Through the innovative work of researchers such as Dr. Crawford, it is clear that the potential for new innovative applications of AnaSpec’s FRET technologies has only just begun to be explored.

Request AnaSpec’s newest FRET Technologies Brochure today.

Products:
390 MMP FRET Substrates
490 MMP FRET Substrates
520 MMP FRET Substrates
580 MMP FRET Substrate

Related Products:

SensoLyte™ MMP Assay Kits
MMP Inhibitors


Table. Publications citing the use of AnaSpec’s SensoLyte™MMP assay kits and their applications.

Cat#

Product

Application

71150

SensoLyte™ 520 MMP-1 Assay Kit

MMP-1 activity measured in hyperhomocysteinemic rats.1

71151

SensoLyte™ 520 MMP-2 Assay Kit

(human) pro-MMP-2 … first activated with 1 mm APMA for 20 min or 2 h…2, 3

…tissue extracts were obtained from nonpregnant, pregnant, and postpartum mice…and a fluorescently labeled substrate specific for MMP2 was used to determine enzyme activity.4

71155

SensoLyte™ 520 MMP-9 Assay Kit

(human) … pro-MMP-9 … first activated with 1 mm APMA for 20 min or 2 h…2, 3

71156

SensoLyte™ 520 MMP-13 Assay Kit

MMP-13 (rat interstitial collagenase) activity was detected at 0 and 7 days using the SensoLyte™ (formerly Enzolyte) 520 MMP-13 assay kit.5

72017

SensoLyte Plus™ 520 MMP-9 Assay Kit

MMP-9 activities of cell [MiaPaCa2 and PANC1 human pancreatic ductal adenocarcinoma cells]or tumor lysates were assessed using the SensoLyte™ (formerly Enzolyte) Plus 520 Enhanced Selectivity MMP-9 activity.6

… genetically altered cells (esophageal squamous) were used to detect MMP-9 enzymatic activity in duplicate using the SenzoLyte Plus MMP-9 assay kit from AnaSpec.7

72019

SensoLyte Plus™ 520 MMP-13 Assay Kit

MMP-13 activity (in RKO- human colorectal carcinoma cell line) cells using the SensoLyte Plus™ 520 MMP-13 Assay.8

71158

SensoLyte™ 520 Generic MMP Assay Kit

General MMP activity (from mouse heart) was measured using the SensoLyte (formerly Enzolyte) 520 Generic MMP assay kit.9

71170

SensoLyte™ 520 MMP Substrate Sampler Kit

Aspergillus culture supernatants, human serum and the mixture of both..(using)..fluorescence resonance energy transfer (FRET) were chosen out of a commercially available protease assay kit comprising 16 different substrates SensoLyte (formerly Enzolyte) 520 matrixmetalloprotease (MMP) substrate sample kit, AnaSpec.10

The activity of MMPs in the conditioned medium and cell lysates (CHO or APP-overexpressed neuroblastoma cells) was determined using SensoLyte™ (formerly EnzoLyte) MMP fluorometric assay kit.11

60580

520 MMP FRET Substrate XIII

The activity of MMP-3 (stromelysin-1) (of rat brain homogenates) was measured fluorometrically using a 5-FAM/QXL520 FRET peptide…12

References:

1. Yi, F. et al. Kidney Int. 70, 88 (2006).
2. Johnson, SL. et al. Chem. Bio. Drug Design 71, 131 (2008).
3. Johnson, SL. et al. Bioorganic Chem. 35, 306 (2007).
4. Wieslander, C. et al. Biol. Reprod.. 10.1095/biolreprod.107.063024 (2007).
5. Arnoczky, SP. et al. Am. J. Sports Med. 35, 763 (2007).
6. Liau, S-S. et al. Cancer Res. 66, 11613 (2006).
7. Okawa, T. et al. Genes & Dev. 21, 2788 (2007).
8. Bai, L. et al. FEBS Lett. 581, 5904 (2007).
9. Errami, M. et al. J. Pharma. Exp. Ther. DOI: 10.1124/jpet.107.133975 (2007).
10. Schaal, R. et al. J. Microbio. Meth. 71, 93 (2007).
11. White, AR. et al. J. Biol. Chem. 281, 17670 (2006).
12. Candelario-Jalil, et al. J. Pharma. Exp. Ther. 323, 488 (2007).