Leveraging AnaSpec’s proprietary detection technologies, AnaSpec FRET-based
MMP substrates were recently used in a double differential in vivo zymography
study at the University of New Brunswick. The successful results demonstrate
the breadth of applications in which AnaSpec’s innovative products can be utilized.
A mixture of QXL™ 570/5-TAMRA-labeled Peptide I incorporating a red fluorophore,
and QXL™ 520/5-FAM-labeled Peptide XIII incorporating a green fluorophore were
used to detect differential breakdown of the two different MMP substrates in
zebrafish. Peptide I was broken down mostly in the maturing myotome boundaries,
whereas Peptide XIII was broken down much more strongly in the notochord sheath
(Courtesy of Dr. Bryan Crawford, Univ. of New Brunswick, Fredericton, NB).
Synthetic FRET peptide substrates provide a reliable method for monitoring
enzyme activity by following substrate cleavage. Through AnaSpec’s innovative
FRET technologies, the SensoLyte™line of assay kits offers detection of MMPs
and other substrates at the industry’s longest wavelengths. Through the innovative
work of researchers such as Dr. Crawford, it is clear that the potential for
new innovative applications of AnaSpec’s FRET technologies has only just begun
to be explored.
Request AnaSpec’s
newest FRET Technologies Brochure today.
Products:
390
MMP FRET Substrates
490
MMP FRET Substrates
520
MMP FRET Substrates
580
MMP FRET Substrate
Related Products:
SensoLyte™
MMP Assay Kits
MMP
Inhibitors
Table. Publications citing the use of AnaSpec’s SensoLyte™MMP assay kits and their
applications.
| Cat# |
Product |
Application |
| 71150 |
SensoLyte™
520 MMP-1 Assay Kit |
MMP-1 activity measured in hyperhomocysteinemic rats.1
|
| 71151 |
SensoLyte™
520 MMP-2 Assay Kit |
(human) pro-MMP-2 … first activated with 1 mm APMA for
20 min or 2 h…2, 3
…tissue extracts were obtained from nonpregnant, pregnant, and postpartum
mice…and a fluorescently labeled substrate specific for MMP2 was used
to determine enzyme activity.4 |
| 71155 |
SensoLyte™
520 MMP-9 Assay Kit |
(human) … pro-MMP-9 … first
activated with 1 mm APMA for 20 min or 2 h…2, 3
|
| 71156 |
SensoLyte™
520 MMP-13 Assay Kit |
MMP-13 (rat interstitial collagenase) activity was detected
at 0 and 7 days using the SensoLyte™ (formerly Enzolyte) 520 MMP-13
assay kit.5 |
| 72017 |
SensoLyte
Plus™ 520 MMP-9 Assay Kit |
MMP-9 activities of cell [MiaPaCa2 and PANC1 human pancreatic
ductal adenocarcinoma cells]or tumor lysates were assessed using
the SensoLyte™ (formerly Enzolyte) Plus 520 Enhanced Selectivity MMP-9
activity.6
… genetically altered cells (esophageal squamous) were used
to detect MMP-9 enzymatic activity in duplicate using the SenzoLyte
Plus MMP-9 assay kit from AnaSpec.7 |
| 72019 |
SensoLyte
Plus™ 520 MMP-13 Assay Kit |
MMP-13 activity (in RKO- human colorectal carcinoma
cell line) cells using the SensoLyte Plus™ 520 MMP-13 Assay.8 |
| 71158 |
SensoLyte™
520 Generic MMP Assay Kit |
General MMP activity (from mouse heart) was measured using
the SensoLyte™ (formerly Enzolyte)
520 Generic MMP assay kit.9 |
| 71170 |
SensoLyte™
520 MMP Substrate Sampler Kit |
Aspergillus culture supernatants, human serum
and the mixture of both..(using)..fluorescence resonance energy transfer
(FRET) were chosen out of a commercially available protease assay kit
comprising 16 different substrates SensoLyte™
(formerly Enzolyte) 520 matrixmetalloprotease (MMP) substrate sample
kit, AnaSpec.10
The activity of MMPs in the conditioned medium and cell
lysates (CHO or APP-overexpressed neuroblastoma cells) was determined
using SensoLyte™ (formerly EnzoLyte)
MMP fluorometric assay kit.11 |
| 60580 |
520
MMP FRET Substrate XIII |
The activity of MMP-3 (stromelysin-1) (of rat brain
homogenates) was measured fluorometrically using a 5-FAM/QXL520 FRET
peptide…12 |
References:
1. Yi,
F. et al. Kidney Int. 70, 88 (2006).
2. Johnson,
SL. et al. Chem. Bio. Drug Design 71, 131 (2008).
3. Johnson,
SL. et al. Bioorganic Chem. 35, 306 (2007).
4. Wieslander,
C. et al. Biol. Reprod.. 10.1095/biolreprod.107.063024 (2007).
5. Arnoczky, SP. et
al. Am. J. Sports Med. 35, 763 (2007).
6. Liau,
S-S. et al. Cancer Res. 66, 11613 (2006).
7. Okawa,
T. et al. Genes & Dev. 21, 2788 (2007).
8. Bai,
L. et al. FEBS Lett. 581, 5904 (2007).
9. Errami,
M. et al. J. Pharma. Exp. Ther. DOI: 10.1124/jpet.107.133975 (2007).
10. Schaal,
R. et al. J. Microbio. Meth. 71, 93 (2007).
11. White, AR.
et al. J. Biol. Chem. 281, 17670 (2006).
12. Candelario-Jalil,
et al. J. Pharma. Exp. Ther. 323, 488 (2007).
|
