SensoLyte™ 520 Cathepsin S Assay Kit – Long-Wavelength


Rapidly becoming the new industry standard for long wavelength FRET protease assay kits, AnaSpec’s SensoLyte™ suite is pleased to introduce the latest member to its growing family – the SensoLyte™ 520 Cathepsin S Assay Kit.

The SensoLyte™ 520 Cathepsin S Activity Assay Kit is a homogeneous assay that can be used to detect the activity of Cathepsin S in biological samples or in purified enzyme preparations. A unique long wavelength 5-FAM/QXL™ 520 FRET peptide substrate is used in this kit. When active Cathepsin S cleaves this FRET substrate, it results in an increase of 5-FAM fluorescence, which can be monitored at Ex/Em = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of cell components and test compounds.

Cathepsin S is a lysosomal cysteine proteinase of the papain family. It plays a major role in the degradation of the invariant peptide chain associated with the major histocompatibility complex and thus affects antigen presentation.1, 2 Since antigen presentation is the key to immune response, Cathepsin S has been validated as an immunomodulatory target.3, 4 Cathepsin S is also involved in several pathologies including atherosclerosis, cancer, obesity and their associated diseases.5-9

Product

Catalog #

SensoLyte™ 520 Cathepsin S Assay Kit *Fluorimetric* NEW

72099



Figure 1. Detection of Cathepsin S with the SensoLyte™ 520 Cathepsin S Assay Kit. This kit can detect 0.1 ng of enzyme (0.03 mUnits).

AnaSpec also provides the SensoLyte 440 Cathepsin S Activity Assay Kit. The substrate used in this kit releases the AMC (7-amino-4-methylcoumarin) fluorophore upon Cathepsin S cleavage. AMC has bright blue fluorescence that can be detected with excitation at 354 nm and emission at 442 nm.

Related Products and Services:

SensoLyte™ 440 Cathepsin S Activity Assay Kit (Ex/Em = 354 nm/442 nm)
SensoLyte™ 520 Cathepsin D Activity Assay Kit (Ex/Em = 490 nm/520 nm)
SensoLyte™ 390 Cathepsin D Activity Assay Kit (Ex/Em = 330 nm/390 nm)
Cathepsin Substrates, Inhibitors & Peptide
Anti-Cathepsin D
b-Amyloid Peptides
SensoLyte™ Secretase Assay Kits

References:

1. Honey, K and AY. Rudensky, Nat. Rev. Immunol. 3, 472 (2003).
2. Hsieh, CS. et al, J. Immunol. 168, 2618 (2002).
3. Gupta, S. et al, Expert Opin. Ther. Targets 12, 291 (2008).
4. Thurmond, RL et al, Curr. Opin. Investi.g Drugs 6, 473 (2005).
5. Sukhova, GK. et al, J. Clin. Invest. 111, 897 (2003).
6. Taleb, S. et al, FASEB J. 19, 1540 (2005).
7. Liu, J. et al, Atherosclerosis 186, 411 (2006).
8. Taleb, S. et al, J. Clin. Endocrinol. Metab. 91, 1042 (2006).
9. Wang, B. et al, J. Biol. Chem. 281, 6020 (2006).