SensoLyte™ 520 HDAC Assay Kit – A New Level of Precision & Sensitivity


Current HDAC assay kits have been based on a short wavelength substrate whose detection is darkened by the presence of autofluorescence interference.  SensoLyte™ welcomes you to the dawn of ultra-sensitivity.

AnaSpec is pleased to introduce the  SensoLyte™ 520 HDAC Activity Assay Kit, the   industry’s longest wavelength assay kit for HDAC detection (Ex/Em = 490 nm/520 nm). With a longer wavelength, the SensoLyte™520 HDAC Activity Assay Kit avoids the autofluorescence interference from cell components and test compounds that is commonly found in traditional HDAC assay kits.

Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases.

The SensoLyte™ 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate.  Without a time-consuming cell extraction step, this assay can be used to measure HDAC activity directly in cell culture in a 96-well plate. This kit can also be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes.

The kit contains: • HDAC 520 substrate • Deacetylated reference standard for calibration • HeLa nuclear extract • Assay buffer • Cell lysis buffer • HDAC developer • Trichostatin A (HDAC inhibitor)

 Product

Size

Catalog #

SensoLyte™ 520 HDAC Activity Assay Kit *Fluorimetric*

1 kit

72084


Figure 1. Signal to background ratio comparison of the SensoLyte 520 HDAC assay kit with the SensoLyte 440 HDAC assay kit.

Figure 2. Trichostatin A inhibition of HDAC activity in HeLa nuclear extract as measured with the long wavelength SensoLyte™ 520 HDAC Activity Assay Kit.

AnaSpec also offers the SensoLyte™ 440 HDAC Activity Assay Kit as a cost-effective solution for moderate sensitivity. This kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using an AMC-based fluorogenic substrate (Ex/Em=354 nm/442 nm).

Product

Size

Catalog #

SensoLyte™ 440 HDAC Activity Assay Kit *Fluorimetric*

1 kit

72083

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Boc-Nε,ε-dimethyl-L-lysine hydrochloride salt

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Fmoc-Lys(Me)3-OH chloride
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Fmoc-No,o-dimethyl-L-arginine (symmetrical)

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Fmoc-No,o-dimethyl-L-arginine (asymmetrical)
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Phosphorylated Serines

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Fmoc-Ser[PO(OBzl)-OH]-OH
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Fmoc-D-Ser[PO(OBzl)-OH]-OH
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