Labeled Streptavidins – High Performance


Both avidin and streptavidin show extraordinary strong affinity to biotin. The dissociation constant (Kd) of the non-covalent interaction between streptavidin and biotin is on the order of 10-15 M. The streptavidin-biotin complex is also highly stable over a wide range of temperatures and pH. As a result, the streptavidin-biotin interaction has been extensively used in molecular biology, immunology, and cellular assays.

AnaSpec is pleased to introduce our latest collection of streptavidins labeled with our patented HiLyte™ Fluor and HiLytePlus™ dyes. They offer brighter fluorescence and better photostability compared to FITC and Cyâ dyes. Streptavidins labeled with fluorescent dyes are widely used for immunofluorescent staining (Fig.1, 2), fluorescence activated cell sorting, in situ hybridization and other biological applications.

Our HiLyte™ Fluor Labeled Streptavidin Sampler kits, available in 2 sizes, contain streptavidins labeled individually with HiLyte™ Fluor. Prepared at optimal fluorophore to streptavidin labeling ratio, these HiLyte™ Fluor streptavidin conjugates exhibit high fluorescence signal and uncompromised biotin binding capability. Every lot has been validated with immunofluorescence staining to guarantee the best performance.

Streptavidins conjugated to classic dyes such as FAM, TAMRA, as well as B-PE, R-PE and APC are also available.

Click here to request additional information

Product

Abs/Em (nm/nm)

Amount

Cat#

Streptavidin, FITC conjugated

494/519

1 mg

60659-FITC

Streptavidin, 5 - FAM conjugated

495/520

1 mg

60664

Streptavidin, HiLyte™ Fluor 488 conjugated

499/523

1 mg

60665

Streptavidin, 5 - TAMRA conjugated

547/574

1 mg

60670

Streptavidin, HiLytePlus™ 555 conjugated

552/567

1 mg

60672-Plus555

Streptavidin, HiLyte™ Fluor 555 conjugated

553/568

1 mg

60666

Streptavidin, HiLyte™ Fluor TR conjugated

591/622

1 mg

60671

Streptavidin, HiLytePlus™ 647 conjugated

649/669

1 mg

60672-Plus647

Streptavidin, HiLyte™ Fluor 647 conjugated

652/669

1 mg

60667

Streptavidin, HiLyte™ Fluor 680 conjugated

678/699

1 mg

60659-H680

Streptavidin, HiLytePlus™ 750 conjugated

751/779

1 mg

60672-Plus750

Streptavidin, HiLyte™ Fluor 750 conjugated

754/778

1 mg

60659-H750

Streptavidin, B - phycoerythrin conjugated

545/575

0.5 mg

60662

Streptavidin, R - phycoerythrin conjugated

565/575

0.5 mg

60669

Streptavidin, crosslinked allophycocyanin conjugated

652/662

0.2 mg

60663

Sampler Kits:

HiLyte™ Fluor Labeled Streptavidin Sampler Kit

3x20 ug

72003-20

HiLyte™ Fluor Labeled Streptavidin Sampler Kit

3x200 ug

72003-200

AP and HRP conjugated Streptavidins:

Streptavidin, alkaline phosphatase conjugated

0.5 mg

60660

Streptavidin, HRP conjugated

1 mg

60668


Related Products

Streptavidin
N - (Biotinoyl) - N'' - (iodoacetyl)ethylenediamine
Protein Labeling Kits


Figure 1. Alpha-tubulin in bovine pulmonary artery endothelial cells were detected with biotin-conjugated mouse anti alpha-tubulin primary antibody, visualized with HiLyte™ Fluor 647 conjugated streptavidin (Cat#60667). Mitochondria were stained with mouse anti Oxphos complex V primary antibody, visualized with HiLyte™ Fluor 488-conjugated goat anti mouse IgG (Cat# 28175-H488). Nucleus was stained with DAPI (Cat# 83210).

Figure 2. Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated phalloidin, visualized with R-phycoerythrin-conjugated streptavidin (Cat#60669). The nuclei were stained with Hoechst 33342 (Cat# 83218).