AnaSpec is pleased to announce the release of the improved AnaTag™ APC,
B-PE and R-PE Labeling Kits.
Labeling of antibodies can
be both tedious and time-consuming. Without the use of proper techniques and
reagents, the conjugation may result in suboptimal or no labeling. AnaSpec’s
improved APC, B-PE and R-PE Labeling Kits contain all the reagents needed for
optimized labeling of antibodies. The phycobiliproteins, modified by SMCC, result
in the creation of SH-reactive moieties, which can easily react with the thiol
groups of target antibody. Since no additional activation is required, the conjugation
process is simple and easy.
are a family of highly soluble and fluorescent proteins derived from cyanobacteria
and eukaryotic algae. Allophycocyanin (APC) is an ultra-sensitive, near-infrared
fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). B-Phycoerythrin
(B-PE), a fluorescent protein from phycobiliprotein family, has a primary absorption
peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.
made of a,
b and g subunits and present as (ab)6g, has broad absorption bands with peaks
at 565 nm (eM =1.96 X 106 M-1cm-1), 498
(eM =1.53 X 106 M-1cm-1),
and 539 nm (eM =1.62 X 106 M-1cm-1. These
phycobiliproteins are significantly
more sensitive and photostable than conventional organic fluorophores. APC, B-PE or R-PE labeled antibodies are used in applications
such as flow cytometry and immunofluorescence.
kits provide all the reagents, purification column needed to label up to 1 mg
of antibody. A detailed step-by-step protocol is also included. APC,
B-PE or R-PE and SMCC activated derivatives of these proteins can also be purchased
here to request additional information
Figure 1. Tubulin in bovine pulmonary artery
endothelial cells were detected with mouse anti-alpha-tubulin primary antibody,
visualized with APC-conjugated goat anti mouse IgG. Nuclei were stained with
DAPI (cat# 83210)
Figure 2. Actins in bovine pulmonary artery
endothelial cells were detected with biotin-conjugated phalloidin, visualized
with R-PE-conjugated streptavidin (cat#60669). Nuclei were stained with Hoechst
33342 (cat# 83218).
Protein Labeling Kits
APC, B-PE and R-PE Products
dye conjugated streptavidins