AnaSpec is pleased to announce the release of the improved AnaTag™ APC, B-PE and R-PE Labeling Kits.

Labeling of antibodies can be both tedious and time-consuming. Without the use of proper techniques and reagents, the conjugation may result in suboptimal or no labeling. AnaSpec’s improved APC, B-PE and R-PE Labeling Kits contain all the reagents needed for optimized labeling of antibodies. The phycobiliproteins, modified by SMCC, result in the creation of SH-reactive moieties, which can easily react with the thiol groups of target antibody. Since no additional activation is required, the conjugation process is simple and easy.

Phycobiliproteins are a family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. Allophycocyanin (APC) is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm. R-Phycoerythrin (R-PE), made of a, b and g subunits and present as (ab)₆g, has broad absorption bands with peaks at 565 nm (e_M =1.96 X 10⁶ M^-1cm^-1), 498 (e_M =1.53 X 10⁶ M^-1cm^-1), and 539 nm (e_M =1.62 X 10⁶ M^-1cm^-1. These phycobiliproteins are significantly more sensitive and photostable than conventional organic fluorophores. APC, B-PE or R-PE labeled antibodies are used in applications such as flow cytometry and immunofluorescence.

AnaTag™ kits provide all the reagents, purification column needed to label up to 1 mg of antibody. A detailed step-by-step protocol is also included. APC, B-PE or R-PE and SMCC activated derivatives of these proteins can also be purchased separately.

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Figure 1. Tubulin in bovine pulmonary artery endothelial cells were detected with mouse anti-alpha-tubulin primary antibody, visualized with APC-conjugated goat anti mouse IgG. Nuclei were stained with DAPI (cat# 83210)

Figure 2. Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated phalloidin, visualized with R-PE-conjugated streptavidin (cat#60669). Nuclei were stained with Hoechst 33342 (cat# 83218).

Protein Labeling Kits

Product	Ex/Em (nm/nm)	Size	Cat #
AnaTag™ APC Labeling Kit NEW	650/660	1 kit	72111
AnaTag™ B-PE Labeling Kit NEW	545, 563/578	1 kit	72112
AnaTag™ R-PE Labeling Kit NEW	565, 498, 539/578	1 kit	72113

APC, B-PE and R-PE Products

Product	Size	Cat #
B - PE (B - Phycoerythrin)	1 mg	82001
CL - APC (Cross Linked - Allophycocyanin)	1 mg	82002
R - PE (R - Phycoerythrin)	1 mg	82004
SMCC Activated B - PE (B - Phycoerythrin) NEW	1 mg	72109
SMCC Activated CL - APC (Cross Linked - Allophycocyanin) NEW	1 mg	72108
SMCC Activated R - PE (R - Phycoerythrin) NEW	1 mg	72110

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