The world’s largest source of b-Amyloid peptides and
related products, AnaSpec is pleased to release our newest collection of ClearPoint™
b-Amyloid
peptides.
ClearPoint™ peptides are AnaSpec’s trademark collection
of heavy or stable isotope labeled peptides and amino acids. These peptides
can be used for the quantitative analyses of b-Amyloid peptides in
biological fluids using mass spectrometry (MS). The
inclusion of internal heavy isotope labeled peptide standards makes mass spectrometry
an absolute quantitation (AQUA) method for the peptide of interest.1
b-Amyloid (1-40) and (1-42) peptides have been
used as biomarkers of Alzheimer's disease (AD). Historically, ELISA assays
have been used in the quantitation of these peptides. However, technical challenges
such as the tendency of b-Amyloid (1-40) and (1-42) peptides to self-aggregate, undergo post-translational
modifications or bind to other proteins result in inaccuracies
in quantitation when using ELISA.2 Oe, et al. reported the use of
[15N]-labeled b-Amyloid peptides as internal standards
using immunoaffinity purified CSF.2
| |
Cat# |
Mass
increase |
Description |
1-letter |
| ClearPoint™ b-Amyloid (1-40),
U-13C, 15N-labeled at Arg & Lys |
63737 |
+26 |
All
the Arginine and Lysines have universally labeled 13C and 15N. |
DAEF-R*-HDSGYEVHHQ-K*-LVFFAEDVGSN-K*-GAIIGLMVGGVV
[R*=R(U-13C6,
U-15N4) &
K*=K(U-13C6,
U-15N2)] |
| ClearPoint™
b-Amyloid (1-42), U-13C,
15N-labeled at Arg & Lys |
63738 |
+26 |
All
the Arginine and Lysines have universally labeled 13C and 15N. |
DAEF-R*-HDSGYEVHHQ-K*-LVFFAEDVGSN-K*-GAIIGLMVGGVVIA
[R*=R(U-13C6,
U-15N4) &
K*=K(U-13C6,
U-15N2)] |
| ClearPoint ™
b-Amyloid (4-42), U-13C,
15N-labeled at Arg & Lys |
63739 |
+26 |
All
the Arginine and Lysines have universally labeled 13C and 15N. |
F-R*-HDSGYEVHHQ-K*-LVFFAEDVGSN-K*-GAIIGLMVGGVVIA
[R*=R(U-13C6,
U-15N4) &
K*=K(U-13C6,
U-15N2)] |
| ClearPoint™
b-Amyloid (1-40), U-13C-labeled
at Leu17 |
63740 |
+6 |
Leu
at position 17 has universally labeled 13C. |
DAEFRHDSGYEVHHQK-L*-VFFAEDVGSNKGAIIGLMVGGVV
[L*=L(U-13C6)] |
| ClearPoint™
b-Amyloid (4-42), U-13C,
15N -labeled at Val12 |
63741 |
+6 |
Val
at position 12 has universally labeled 13C and 15N. |
FRHDSGYE-V*-HHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
V*=V(U-13C5,
U-15N) |
| ClearPoint™
b-Amyloid (8-42), U-13C,
15N -labeled at Val12 |
63742 |
+6 |
Val
at position 12 has universally labeled 13C and 15N. |
SGYE-V*-HHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
V*=V(U-13C5,
U-15N) |
Samples of
other newly released b-Amyloid GO™
Peptides:
| [Cys26] - beta - Amyloid (1 - 40), S26C beta - Amyloid
(1 - 40) NEW |
1 mg |
63671-1 |
[Cys26] - beta - Amyloid (1 - 42), S26C beta - Amyloid
(1 - 42) NEW |
0.5 mg |
63672-05 |
| [Cys40] - beta - hairpin (BHA), Protein G B1 Domain (41
- 56) NEW |
1 mg |
62533 |
| [Cys7] - Beta - Amyloid (1 - 40) NEW |
1 mg |
62815-1 |
| [Cys7] - Beta - Amyloid (1 - 40) NEW |
0.5 mg |
62815-05 |
| [Gln22] - beta - Amyloid (15 - 23) NEW |
1 mg |
62892 |
| [Gln22] - beta - Amyloid (1 - 40), Dutch Mutation |
0.5 mg |
61261 |
| [Gly21] - beta - Amyloid (1 - 40), A21G, Flemish mutation NEW |
0.5 mg |
62150 |
| [Gly22] - beta - Amyloid (1 - 40), Arctic Mutation |
0.5 mg |
61262 |
| [Gly22] beta - Amyloid (1 - 42), Arctic Mutation NEW |
0.5 mg |
61967-05 |
| [Lys22] Beta - Amyloid (1 - 42), Italian Mutation NEW |
0.5 mg |
62148 |
| [Gly30, Cys31] beta - Amyloid (13 – 31) |
1 mg |
61800 |
| [Gly35, Asp37] - Beta - Amyloid (1 - 42) NEW |
0.1 mg |
62832-01 |
| [Gly35, Asp37] - Beta - Amyloid (1 - 42) NEW |
0.5 mg |
62832-05 |
| [Ile34] Beta - Amyloid (25 – 34) |
1 mg |
61077-1 |
| [Ile34] Beta - Amyloid (25 – 34) |
5 mg |
61077-5 |
| [Leu35] -
beta - Amyloid (1 - 42) NEW |
0.5 mg |
62143 |
| [Leu35] - Met - beta - Amyloid (1 – 42) |
0.1 mg |
61957-01 |
World’s largest
collection of b-Amyloid and Related Peptides
Click
here to request the new Alzheimer’s Research Brochure
b-Amyloid on sepharose available. Send email to service@anaspec.com for more information.
Related
Products:
Labeled Amyloid Peptides
SensoLyte™ 520 Beta-Secretase Assay Kit
SensoLyte™ 520 Alpha-Secretase Assay Kit
SensoLyte™ Cathepsin
Assay Kits
Anti-Tau
Antibodies – phospho and non-phosphorylated
DHL™
fluorescent b-Amyloid (1-40) & (1-42)
Sampler Kits
Amyloid
Vital Dyes
References
1. Harding, S. (ed.) 2007. Biotechnology & Genetic Engineering
Reviews. Nottingham Univ. Press, Nottingham.
2. Oe, T. et al. Rapid Comm. Mass Spec. 20, 3723 (2006).
|
